中文摘要：

目的：研究 rMBP - NAP 在肝癌 H22荷瘤小鼠模型中的肿瘤生长抑制作用以及 IL -12/ IL -23在其发挥抗肿瘤作用中的角色。方法 BABL/ c 小鼠左前肢腋下皮下注射2×10^6个 H22肿瘤细胞，建立荷瘤小鼠模型，荷瘤后第2天将小鼠随机分为两组，PBS 组和 rMBP - NAP 组，并通过皮下瘤旁注射给药的方式进行治疗，期间监测肿瘤体积。取荷瘤小鼠脾脏制成单细胞悬液，分为对照组和抗体阻断组，同时加入丝裂霉素 C 处理过的 H22细胞和 rMBP - NAP 与脾细胞共培养，抗体阻断组中分别加入 anti - IL -12抗体和 anti - IL -23抗体，对照组中加入同型抗体。于培养3 d 和7 d 后检测 IFN -γ的表达。结果相对于 PBS 组，rMBP - NAP 治疗组小鼠的肿瘤生长显著减缓。rMBP - NAP 刺激后，荷瘤小鼠脾细胞分泌大量 IFN -γ，共培养3 d 和7 d 后，IFN -γ表达持续累积。Anti - IL -12抗体和 anti - IL -23抗体阻断后，均可导致 IFN -γ的表达量显著性下降。结论 rMBP - NAP 能够显著性抑制 H22荷瘤小鼠肿瘤的生长。

英文摘要：

Objective To study the antitumor effect and action mechanism of rMBP - NAP in hepatic cancer H22 bearing mice. Methods 2 ×10^6 H22 hepatoma cells were injected subcutaneously into the left flank of BABL/ c mice. On the next day, mice were divided into two groups and treated with PBS and rMBP - NAP,respectively. The tumor growth was monitored every day. The splenocytes from tumor bearing mice were cocultured with MMC - treated H22 tumor cells in the presence of rMBP -NAP,and anti - IL - 12 or anti - IL - 23 antibodies were added immediately. The secretion of IFN - γ in the supernatant were detected with ELISA assay 3 and 7 days later. Results Compared to PBS - treated mice,rMBP - NAP treatment significant-ly inhibited tumor growth. Moreover,the secretion of IFN - γ of splenocytes from tumor bearing mice was significantly in-creased with stimulation of rMBP - NAP in vitro. And it decreased significantly after addition of anti - IL - 12 or anti - IL -23 antibodies 3 and 7 days later. Conclusion Immunoregulatory agent rMBP - NAP can significantly inhibit hepatic canc-er H22 growth in mice.