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不同磷源形态对中肋骨条藻和东海原甲藻生长及磷酸酶活性的影响

ISSN号：0250-3301
期刊名称：《环境科学》
时间：0
分类：X55[环境科学与工程—环境工程]
作者机构：[1]中国科学院海洋研究所海洋生态与环境科学重点实验室,青岛266071, [2]中国科学院研究生院,北京100049
相关基金：基金项目：中国科学院知识创新工程重要方向项目（KZCX3-SW-232）;国家重点基础研究发展规划（973）项目（2007CB407305）;中国科学院知识创新工程重要方向项目（KZCX2.YW-208）;创新研究群体基金项目（40821004）

关键词：中肋骨条藻, 东海原甲藻, 有机磷化合物, 磷酸酶, Skeletonema costatum , Prorocentrum donghaiense , dissolved organic phosphorus, phosphatase activity

中文摘要：

研究了磷酸二氢钠（NaH2PO4）、三磷酸腺苷二钠盐（adenosine disodium triphosphate,ATP）、6-磷酸葡萄糖（glucose 6-phosphate,G-6-P）和β-甘油磷酸钠（sodium-βglycerophosphate,G-P）对中肋骨条藻（Skeletonema costatum）和东海原甲藻（Prorocentrumdonghaiense）的生长以及磷酸酶活性的影响.结果发现,中肋骨条藻和东海原甲藻均既可利用无机磷酸盐又能利用有机磷化合物生长繁殖,且有机磷化合物的作用比无机磷酸盐的作用稍高.对于中肋骨条藻,8 d后的细胞密度分别为48×10^4（NaH2PO4）、73×10 4（ATP）、63×10^4（G-6-P）和54×10^4（G-P）cells/mL;对于东海原甲藻,10 d后4种磷源形态下的细胞密度分别为8.7×10^4、15.5×10^4、12.4×10^4和9.5×10^4cells/mL.2种藻在4种形态磷源下的磷酸酶活性在前3～4 d均呈下降趋势,而后变化不同.以NaH2PO4为磷源的处理组,2种藻的碱性磷酸酶（alkaline phosphatase,AP）和酸性磷酸酶（acid phosphatase,AcP）活性均呈现升高趋势;3种有机磷处理组比较,对于中肋骨条藻,ATP和G-6-P为磷源的处理组酶活性较低且AP活性无明显变化趋势,AcP活性在试验后期呈微弱的升高趋势,以G-P为磷源的处理组酶活性最高且有升高趋势,至第8 d时,以ATP、G-6-P和G-P为磷源处理组的AP活性分别为0.004×10^-5、0.014×10^-5和0.029×10^-5U/cell,AcP活性分别为0.006×10^-5、0.011×10^-5和0.018×10^-5U/cell,对于东海原甲藻,酶活性变化趋势与中肋骨条藻相同,至第10 d时,3种磷源处理组的AP和AcP活性顺序也为ATPSN 0250-3301

英文摘要：

The effects NaH2PO4, adenosine disodium triphosphate （ATP）, glucose 6-phosphate （G-6-P） and sodium β-glycerophosphate （G-P） on the growth and phosphatase activity of Skeletonema costatum and Prorocentrum donghaiense were studied. The results showed that both species could utilize both dissolved inorganic phosphate （DIP） and dissolved organic phosphorus （DOP）, and DOP had more effects on the growth of two species than DIP. For S. costatum, after 8 days, the cell abundances of the four treatments （NaH2PO4, ATP, G-6-P and G-P） were 48 ×10^4 , 73 ×10^4 , 63 ×10^4 and 54×10^4cells/mL, respectively; For P. donghaiense, after 10 days, the cell abundances of the four treatments were 8.7 ×10^4 , 15.5 ×10^4 , 12.4 ×10^4 and 9.5 ×10^4cells/mL, respectively. On the first 3-4 days, the phosphatase activity of all treatments of the two species showed a decreasing trend, but different changes were observed for the different phosphorus substrate treatments in latter days. For the Nail2 PO4 treatment, both the AP and AcP activity of two species increased from the fifth day onwards. For S. costatum, the AP activity of the ATP and G-6-P treatment groups showed no obvious changes and AcP activity had a slight increase from the fifth day to the eighth day, while the activity of G-P treatment had highest phosphatase activity which increased from the fifth day on. At the end of the experiment, the AP activity of the three DOP treatment groups （ATP, G-6-P and G-P） was 0.004 ×10^-5 , 0.014 ×10^-5 and 0.029 ×10^-5 U/cell, respectively, and the AcP activity was 0.006 ×10^-5 , 0.011 ×10^-5 and 0.018 ×10^-5 U/cell, respectively. For P. donghaiense, both the AP and AcP activity of the three DOP treatments had similar trends, i.e., ATP 〈 G-6-P 〈 G-P. Under the same nutrient conditions, S. costatum had a much higher phosphatase activity and could absorb P from the environment much faster than P. donghaiense .