中文摘要：

利用细胞培养、原位杂交、免疫组化、图像分析等技术研究缺氧与肉鸡肺动脉平滑肌细胞癌基因产fos和产myc表达的关系，进一步阐明缺氧与以肺动脉增殖重塑为特征的肉鸡腹水综合征的关系。结果显示，缺氧显著引起肺动脉平滑肌细胞内癌基因产fos和c—myc mRNA的表达（c-fos mRNA：常氧组为144．6±20．2，缺氧组为198．1±32．8，P〈0．01；c-myc mRNA：常氧组为125．4±18．8，缺氧组为167．1±22．4，P〈0．01）。缺氧显著引起肺动脉平滑肌细胞内癌基因c-los和c-myc蛋白的表达（c-los蛋白：常氧组为150．9±33．2，缺氧组为225．95=37．0，P〈0．01；c-myc蛋白：常氧组为162．1±28．5，缺氧组为228．8±33．4，P〈0．01）。结果表明缺氧能够明显诱发肉鸡肺动脉平滑肌细胞内癌基因产fos、c-myc的转录和表达，是启动肺动脉平滑肌细胞增殖的重要原因。本研究阐明缺氧是以肺动脉重塑为特征的肉鸡腹水综合征的主要诱因。

英文摘要：

This experiment aimed to study the relationship between hypoxia and expression of c-fos,c-myc in broiler＇s pulmonary arterial smooth muscle cell （PASMC）. The expression of c-fos,c-myc were observed by in situ hybridization, immunohistochemistry and image analysis methods. The results showed that hypoxia can significantly increase c-fos and c-my mRNA expression（c-fos mRNA. 144.6＋20.2 in normal oxygen group, 198.1＋32.8 in hypoxia group,P 〈0. 01; c-myc mRNA： 125.4±18.8 in normal oxygen group, 167.1＋22.4 in hypoxia group,P〈0.01）. Hypoxia can significantly increase c-fos and c-myc protein expression（c-los protein： 150. 9±33.2 in normal oxygen group, 225.9＋37.0 in hypoxia group; c-myc protein： 162.1± 28.5 in normal oxygen group, 228.8 ＋ 33.4 in hypoxia group, P〈0.01）. The results indicated that hypoxia play an important role in the PASMC proliferation. As pulmonary arterial remoulding were the main characters of ascites syndrome（AS）, so this experiment indicated that hypoxia is the important cause of AS.