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中文摘要：
2013年福建省某鹅场发生一起以肝脏和脾脏均有灰白坏死点（肝白点）为典型病变特征的急性传染病。本研究利用10日龄非免疫番鸭胚，从病死雏鹅肝脏中分离到1株病毒。病毒分离株没有血凝活性，其ELD50为10-3.25/0.2 mL，可被鹅呼肠孤病毒高免血清特异性中和。利用鹅呼肠孤病毒特异性检测引物对FJ-06株病毒尿囊液进行RT-PCR检测，可扩增到约572 bp特异性目的条带。将RT-PCR产物进行克隆测序，Blast分析表明，克隆的基因片段为鹅呼肠孤病毒δC蛋白基因片段。结果表明成功分离到1株鹅呼肠孤病毒。
英文摘要：
An acute infectious disease occurred in a gosling flock in 2013 in Fujian Province. Gray necrotic spots were observed in livers and spleens. Livers collected from dead goslings were homologized and inoculated into 10-day-old Muscovy duck embryos for virus isolation. The isolated virus had no hemagglutinating activity for chicken and guinea pig red blood cells. When duck embryos were inoculated with the isolated virus, the ELD50 was determined to be 10-3.25/0.2 mL. In addition, the isolated virus was neutralized with the hyper-immune serum from vaccinated geese. Viral RNA was amplified from the isolated virus using RT-PCR with specific target primers. A fragment of 572 bp was obtained, then sequenced and analyzed. The results confirmed that the isolated virus was a reovirus.
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