中文摘要：

大豆皂甙具有多种药理作用，而对大豆制品中各类皂甙的定量分析是研究大豆皂甙药理作用的基础。采用高效液相色谱-差示折光（HPLC—dRI）检测法测定单糖链大豆皂甙的含量。色谱条件为：ODS—AM-303色谱柱（YMC，4．6mm×250mm，5μm），柱温40℃，含0．19／6三氟乙酸的乙腈-水（40：60）为流动相，流速1mLmin^-1。Ba、Bb、Bd、Be、ag和8g分别在2．24～11．2、2．35～11．8、1．58～7．92、2．01～10．1、1．38～6．88和1．62～8．12μg范围内线性关系良好，平均回收率分别为92．5％、93．8％、94．1％、95．8％、93．4％和94．2％，RSD为3．22％、3．18％、4．01％、3．53％、4．07％和4．28％。该方法准确度高，重现性好，适用于大豆皂甙样品中各类皂甙的测定。

英文摘要：

Soyasaponins have many pharmacological effects. The determination of soyasaponins contents is the basis of the pharmacological experiments. In the current experiment mono-desmoside soyasaponins in soybean sample were detected by a high performance liquid chromatographicdifferential refractive index detective （HPLC-dRI） method. The chromatographic condition was as fellows：ODS-AM-303 column （YMC,4.6 mm 250 mm,5μm） with the temperature of 40℃ ,acetonitrile-water （40 ： 60） containing 0.1% trifluoroacetic acid as mobile phase with the flow rate of 1 mL min^-1. The method was proved to be linear in ranges of 2.24-11.2,2. 35-11.8,1.58-7.92,2.01-10.1,1.38-6.88 and 1.62-8.12 μg for Ba,Bb,Bd,Be,ug and 13g,respectively;the recoveries were 92.5%,93.8%,94.1%,95.8%,93.4% and 94.2%o with RSD of 3.22%,3.18%, 4.01%,3.53%,4.07% and 4.28% for Ba,Bb,Bd,Be,αg and βg,respectively. The method is accurate and reproducible,and suitable for the determination of mono-desmoside soyasaponins.