目的:确定地黄益智颗粒中石菖蒲、盐益智挥发油的提取和包合工艺。方法:采用正交试验设计,以挥发油提取量为指标优选挥发油的水蒸气蒸馏提取工艺;以包合物收率和挥发油包合率为指标优选β-环糊精包合工艺。结果:最佳提取工艺为加水量8倍,浸泡0.5 h,提取8 h;最佳包合工艺为采用研磨法,挥发油∶β-环糊精1∶4,加水量为环糊精的1.5倍,研磨时间60 min。结论:石菖蒲、盐益智混合挥发油的提取率大于1.5%,挥发油的包合率大于95%。挥发油的提取及包合工艺简单易行,有效保留了地黄益智颗粒中的挥发油成分。
Objective:To obtain the extraction technique of mixed volatile oil from Acorus tatarinowii and Alpinia oxyphlla in Dihuang Yizhi granules and their β-CD inclusion technology.Method:The orthogonal design was applied.The yield of volatile oil was used to evaluate the extraction process by steam distillation method.The inclusion yield and volatile oil inclusion rate were used to determine the inclusion process.Result:The optimized extraction progress parameters were as follows:water volume was 10-fold,extracted time was eight hours,and soaking time was 0.5 h.The optimized inclusion progress parameters were as follows:the rate of oil:β-CD(g:mL) was 1:4,water volume was 1.5-fold,and grinding time was 60 minutes.Conclusion:The yield rate of volatile oil is greater than 1.5% and volatile oil inclusion rate is more than 95%.The optimum extraction process and inclusion process were simple and practicable,which can effectively retain the volatile oil in Dihuang Yizhi granules.