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Species delimitation in the green algal genus Codium （Bryopsidales） from Korea using DNA barcoding

ISSN号：0253-505X
期刊名称：《海洋学报：英文版》
时间：0
分类：S718.54[农业科学—林学] S336[农业科学—作物遗传育种;农业科学—农艺学]
作者机构：[1]Department of Biology, Jeju National University, Jeju 690-756, Korea
相关基金：Foundation item： A grant from the National Institute of Biological Resources （NIBR）, funded by the Ministry of Environment （MOE） of the Republic of Korea under contract No. 2013-02-013. The authors thank all members of the Molecular Phylogeny of Marine Algae Laboratory at leju National University.

关键词：物种多样性, 海洋绿藻, 条形码, DNA, 韩国, RBCL基因, 形态学鉴定, 松, Codium, DNA barcoding, rbcL, Species delimitation, tufA

中文摘要：

Codium，最大的海洋的绿海藻的类之一，是困难的仅仅基于词法鉴定精确地限定种边界。DNA barcoding 是为区别海草的种类的一个强大的工具。质体延伸因素 TU 石灰华被看作制造者由于普遍性和快速的进化率比 rbcL 基因执行绿海藻的种类的 DNA barcoding。我们从 Jeju 岛进行了 DNA barcoding 申请到 Codium 标本，克服词法鉴定的限制并且证实种类差异的朝鲜。由于使用石灰华标记，我们最新产生了 55 石灰华 barcodes 解决八种。石灰华标记展出了 6.1%21.8% 种间的分叉，比 rbcL exon 的差距宽 1， 3.5%11.5% 。1 Codium 定序的 rbcL exon 的分子的分析揭示了象在五个种系发生的组分开的石灰华分析一样的八不同的种。用石灰华标记的类 Codium 的 DNA barcoding 是更有用的克服词法鉴定的限制，并且这是更潜在的揭示神秘种类并且独自比 rbcL 分析在亚种之中解决关系。包括为在西北的太平洋的类 Codium 的形态学的石灰华 barcoding 和 rbcL 分析的补充将为发现种差异并且解决种系发生的关系给更可靠的成就。

英文摘要：

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU （tufA） is considered as maker to perform DNA barcoding of green algal species than rbcL gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tufA marker, we newly generated fifty-five tufA barcodes to resolve eight species. TufA marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbcL exon 1, 3.5%–11.5%. Molecular analysis of rbcL exon 1 sequences of Codium revealed eight distinct species like tufA analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tufA marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbcL analysis alone. The complement of tufA barcoding and rbcL analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.