中文摘要：

本研究旨在克隆鸡（Gallus gallus）脂滴包被蛋白基因（Perilipin1）的c DNA序列,并检测其在鸡前脂肪细胞分化过程中的亚细胞定位。本研究以7周龄肉鸡腹部脂肪组织为材料,采用RT-PCR和RACE的方法扩增并克隆了鸡Perilipin1基因的5＇UTR、CDS和3＇UTR片段,分析了该基因的结构;以鸡原代前脂肪细胞为材料,利用免疫荧光及激光共聚焦技术,在诱导分化的不同时间点（12~120 h）,检测了鸡Perilipin1在前脂肪细胞中的表达位置。结果表明,本研究获得的鸡Perilipin1基因5＇UTR、CDS和3＇UTR序列总长度为2 379 bp,该基因由9个外显子、8个内含子组成（ATG位于第二外显子上）;在鸡原代前脂肪细胞诱导分化过程中,Perilipin1始终包被在脂滴周围。综上,本研究成功克隆了鸡Perilipin1基因的完整c DNA序列并确定了Perilipin1在鸡前脂肪细胞分化过程中与脂滴的空间位置关系,该结果为深入开展鸡Perilipin1基因的功能研究,揭示鸡脂肪代谢的分子遗传机理提供了重要的理论依据。

英文摘要：

Surrounding the membrane surface of lipid droplets, Perilipin1 plays important roles in the formation and maturation of lipid droplets, and regulation of fat mobilization（lipolysis）. Considered as one of the important marker genes of adipocyte differentiation, many Perilipin1 studies in mammals have been carried out, while in chickens（Gallus gallus） relatively few. The objectives of the present study were to clone full length m RNA of chicken Perilipin1 gene, and analyze its subcellular location during the progression of chicken preadipocyte differentiation. We extracted total RNA of abdominal adipose tissue from chickens at 7weeks of age. RT- PCR and rapid amplification of c DNA ends（RACE） methods were used to clone the 5＇-untranslated region（5＇- UTR）, the coding domain sequence（CDS） and the 3＇- untranslated region（3＇- UTR）sequences of chicken Perilipin1 gene, and then the gene structure was analyzed. The subcellular localizations of Perilipin1 in chicken preadipocyte was examined by immunofluorescence confocal microscopy at differenttime points（12~120 h）. Results showed that the whole m RNA length of chicken Perilipin1 gene was 2 379bases（bp）, consisted of nine exons and eight introns（with the start codon ATG located in the second exon）.The CDS length of chicken Perilipin1 gene was 1 551 bp, encoding 517 amino acids, and lengths of 5＇-UTR and 3＇ UTR were 94 and 733 bp, respectively. Sequences have been submitted to NCBI database, and assigned the serial number（Accession No. GU327532）. In addition, we performed the multiple alignment for the chicken Perilipin1 CDS sequence with mouse（NM_175640） and human（NM_001145311） sequences deposited in NCBI database by the Clustalw software（http：//www.genome.jp/tools/Clustalw/）, and obtained the homology percentages, 38% and 41%, respectively. Therefore, sequence divergence at nucleotide level was quite large, suggesting that the role of chicken Perilipin1 in adipocye differentiation may not be fully consiste