中文摘要：

目的构建多个人肝再生增强子（hALR）的原核表达系统进行原核表达、鉴定及筛选，得出理想的系统用于进一步的研究。方法构建4个重组表达质粒pET28a（＋）／hALR、pET23a／hALR、pGEX-5x-1／hALR、pGEX-5x-2／hALR，分别转化3种宿主菌，诱导表达重组hALR蛋白，用SDS-聚丙烯酰胺凝胶电泳和Western blotting鉴定重组蛋白。结果双酶切和DNA测序证实hALR cDNA正确插入4种表达载体并成功转化宿主菌，仅pET28a（＋）／hALR的BL21（DE3）菌株成功表达相对分子质量为23kDa的重组蛋白。结论筛选得出人肝再生增强子的原核表达系统，成功表达并鉴定重组hALR蛋白。

英文摘要：

Objective As to obtain a perfect prokaryotic expression system for lucubration, several prokaryotic expression systems of human augmenter of liver regeneration （hALR） were constructed to conduct prokaryotic expression, identification and filtration. Methods Four recombinant expression plasmids pET28a （ ＋ ） /hALR, pET23a/hALR, pGEX-Sx-1/hALR, pGEX-Sx-2/hALR were constructed and transformed respectively into three host bacteria, Escherichia coliBL21 （DE3）, JM109 and DHSa, to express the recombinant hALR protein which was then determined by SDS-PAGE and Western blotting. Results The correct insertion site in the four expression vectors were confirmed by restriction endonuclease digestion and the correct DNA sequence of the insertion fragment was determined by DNA sequencing, pET28a （ ＋ ） /hALR in BL21 （DE3） was the only recombinant plasmid which successfully expressed a recombinant protein, of which the molecular weight was about 23kDa. Conclusions The prokaryotic expression system of human augmenter of liver regeneration by filtration successfully expressed and identified recombinant hALR protein.