中文摘要：

目的:建立高效液相色谱(high performance liquid chromatography,HPLC)法同时测定金针菇中麦角甾醇和22,23-二氢麦角甾醇含量。方法:采用Inertsil ODS-SP(250 mm×4.6 mm,5μm)色谱柱同时测定上述2种成分,流动相为甲醇,流速1.0 mL·min-1,检测波长为282 nm,柱温25℃。结果:麦角甾醇和22,23-二氢麦角甾醇的进样量在0.025~1.5μg范围内呈良好线性关系(r=0.999 9);平均回收率(n=6)分别为104.6%[相对标准偏差(RSD)=0.89%],103.5%(RSD=3.34%)。结论:该方法简便,快速,准确,适用于测定金针菇中麦角甾醇和22,23-二氢麦角甾醇的含量。

英文摘要：

Objective:To develop an efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.Methods:Chromatographic separation was achieved on an Inertsil ODS-SP (250 mm × 4.6 mm,5 μm) analytical column using a mobile phase of 100％ methanol at 1.0 mL · min-1 The sterol content was detected at 282 nm.The temperature of column was 25 ℃.Results:The established linearity range for ergosterol and 22,23-dihydroergosterol was 0.025-1.5 μg (r =0.999 9).The average recoveries (n =6) were 104.6％ with RSD 0.89％,103.5％ with RSD 3.34％,respectively.Conclusion:The established HPLC method was simple,rapid and reliable for the separation and simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.