中文摘要：

目的观察中药清热活血解毒复方对人永生化角质形成细胞株（HaCdT）增殖、凋亡和细胞周期的影响，探讨其治疗银屑病可能的作用机制。方法将不同浓度的清热活血解毒复方水提物分别作用于体外培养的HaCaT细胞，采用CCK-8法检测药物对细胞增殖的抑制作用及药物的有效浓度；采用倒置显微镜观察药物处理前后细胞的形态学改变；流式细胞术检测细胞周期的变化及凋亡率。结果清热活血解毒复方以时间和浓度依赖性方式抑制HaCaT细胞增殖，作用24、48h其半抑制浓度（IC50）分别为23．40、20．24mg／mL。细胞形态学和流式细胞仪检测发现约物以浓度依赖性方式干扰细胞周期，诱导细胞凋亡，细胞分裂周期阻滞于G0／G1期。结论清热活血解毒复方可能通过抑制HaCaT细胞增殖、诱导捌亡、影响细胞周期而发挥治疗银屑病的作用。

英文摘要：

Objective To investigate the effect of Qingre Huoxue Jiedu Compound Formula on proliferation, apoptosis, and cell cycle of HaCat cell in vitro and its possible action mechanism in the treatment of psoriasis. Methods The cultured HaCat cells in vitro were exposed to the water extract of Qingre Huoxue Jiedu Compound Formula with different concentrations, the inhibition on cell proliferation and the effective concentrations of compound formula were measured by CCK-8 assay. Morphological changes before and after the treatment was observed under the inverted microscope. Cell cycle and apoptosis rate were tested by flow cytometry. Results Qingre Huoxue Jiedu Compound Formula inhibited the proliferation of HaCat cells in a time and concentration-dependent mode. The IC50 of it for 24 h and 48 h were 23.40 mg/mL and 20. 24 mg/mL respectively. Cell morphological changes and flow cytometry analysis demonstrated that the Compound Formula could disturb the cell cycle, induce cell apoptosis and stop the cell division at G0/G1 phase in a concentration -dependent manner. Conclusion Qingre Huoxue Jiedu Compound Formula may treat psoriasis by inhibiting proliferation, inducing apoptosis and affecting cell cycle of HaCat cells.