中文摘要：

目的：检测 ING4对甲状腺癌 SW579细胞周期的影响及其机制。方法：利用软琼脂克隆形成实验检测细胞增殖，并利用 PI 染色和 Cldu/ Idu 双染检测细胞周期变化。Western blot 技术检测周期相关蛋白的变化。结果：ING4可以明显抑制 SW579细胞增殖，并且导致其 S 早期阻滞。ING4处理后细胞 P53表达明显升高，同时 PhosPho - S345- Chk1和 PhosPho - T68- Chk2等细胞周期相关蛋白表达水平升高。结论：ING4可导致 SW579细胞出现 S 早期阻滞，P53表达明显升高，细胞周期相关蛋白表达水平升高，但是三者关系有待进一步研究。

英文摘要：

Objective：To study the effects and mechanisms of ING4 on cell cycle of thyroid cancer cell SW579. Methods：The Proliferative ratio of SW579 cells after treated with ING4 was detected by soft agarose assay. We detec-ted the changes of cell cycle after treated with ING4 by PI staining and Cldu/ Idu double staining. The cell cycle relat-ed Proteins were analyzed by Western blot. Results：ING4 could inhibit the Proliferation and induced S Phase early stage arrest in SW579 cells. The levels of cell cycle related Proteins,P53,PhosPho - S345 - Chk1 and PhosPho - T68- Chk2,were higher in treated cells than untreated ones. Conclusion：ING4 could induce S early stage arrest in SW579 cell,uP - regulate P53 exPression and cell cycle related Proteins. eowever,the relationshiP among ING4,P53 and cell cycle related Proteins by activating P53 exPression was needed further studies.