中文摘要：

从药用蕨类植物蛇足石杉叶中分离得到53株内生真菌,在马铃薯葡萄糖液体培养基（PDB）中发酵后对内生真菌发酵提取物液进行TLC、HPLC和ESI-MS检测,结果表明菌株LF40为产石杉碱甲内生菌株,含量达80.1μg/gdcw,并具有体外乙酰胆碱酯酶抑制活性;运用形态学及ITS-rDNA序列分析方法对菌株LF40进行鉴定,确定为黄曲霉Aspergillus flavus LF40。

英文摘要：

A total of fifty-three endophytic fungal isolates were obtained from fresh leaves of Huperzia serrata,a traditional Chinese medicinal plant.The strain LF40 was found to produce huperzine A（HupA） in potato dextrose broth（PDB） with a yield of 80.1μg /gdcw,based on TLC,HPLC and ESI-MS detection.The inhibition effects of TLC purified fungal HupA from strain LF40 was similar to authentic HupA.Morphological characteristics and internal transcribed spacer（ITS） sequences show that the strain LF40 was Aspergillus flavus LF40.