中文摘要：

目的 观察骨髓干细胞是否可以向肾祖细胞转分化,成为肾脏祖细胞库的肾外来源;验证粒细胞集落刺激因子（G-CSF）是否可以促进骨髓干细胞向肾脏祖细胞的转分化,提高肾脏修复的效能.方法 6周龄全身表达绿色荧光蛋白（GFP）的C57BL/6J转基因小鼠提供骨髓,6-8周龄同种无荧光标记的C57BL/6J小鼠40只作为骨髓受体.骨髓移植前,受体小鼠接受致死剂量的γ放射线137Cs照射,骨髓重建情况经流式细胞仪检测确认.骨髓重建完毕后所有小鼠均接受单侧肾脏缺血再灌注损伤.干细胞动员效果及向肾脏归巢情况经流式细胞仪检测鉴定.损伤4、8周后取肾脏标本行免疫荧光组织化学染色,观察骨髓来源的肾脏祖细胞数以及骨髓细胞在微血管形成中的作用.损伤4周后通过组织切片免疫荧光组织化学方法观察并计数微血管细胞数.结果 G-CSF动员1 d后,分别为CD29、CD34、Sca-1、c-Kit、Flk-1阳性的干细胞占外周血非红系细胞的比例均高于对照组（P〈0.05）.损伤4周后,G-CSF动员组的肾脏中,骨髓来源并且分别表达Sca-1/GFP、CD29/GFP的干细胞的比例均高于对照组（P〈0.05）;在损伤4周及8周后,肾脏切片免疫荧光组织化学显示G-CSF动员肾脏中骨髓来源的肾祖细胞即Sca-1/GFP双阳性的细胞数量高于对照组.损伤4周后,动员组肾脏中表达CD31的微血管密度高于对照组（P〈0.05）.损伤4周后肾脏组织中存在CD105/GFP及α-SMA/GFP双阳性的细胞.结论 ①骨髓干细胞可以转分化为器官特异性干细胞-肾脏祖细胞;②G-CSF可以加速这一转分化的过程,并使损伤肾脏得到更好的修复.

英文摘要：

Objectives To investigate if bone marrow derived stem cells can transdifferentiate into renal progenitor cells, explore if the moblilization by G-CSF can promote the transdifferentiation of bone marrow derived stem cells into renal progenitor cells.Methods We transplanted BM cells from transgenic mice, expressing enhanced green fluorescent protein （EGFP） into wild-type irradiated recipients.Before bone marrow transplantation, the receptor mice received lethal radiation by 137Cs.Bone marrow reconstruction was confirmed by flow cytometry.After bone marrow reconstruction, all mice were treated with unilateral renal ischemia reperfusion injury.Peripheral blood stem cell mobilization and homing to kidney were confirmed by flow cytometry.Following hematological reconstitution and ischemia-reperfusion （I/R）, Sca-1 and c-Kit positive renal stem cells in kidney were evaluated by immunostaining and flow cytometry analysis.The number of microvascular cells in kidney tissue were evaluated by immunostaining.Results The number of stem cells increased significantly in peripheral blood after G-CSF administration.BM-derived cells can contribute to the Sca-1＋ or c-Kit＋ renal progenitor cells population in kidney.Furthermore, G-CSF administration increased capillary density of I/R injured kidneys.Conclusions These findings indicate that BM derived stem cells can give rise to cells that share properties of renal resident stem cell.Moreover, G-CSF mobilization can enhance this effect.