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中文摘要：
目的测定梅花入骨丹中没食子酸和槲皮素的含量。方法用HPLC法，以SinoChrom C18（4．6mm×250mm，5μm）为色谱柱，流动相为乙腈-0．2％磷酸溶液，流速为1mL／min，检测波长为255nm，柱温为30℃，梯度洗脱。结果没食子酸在0．162-3．24μg，槲皮素在0．002052-0．041μg内呈良好的线性关系，没食子酸平均回收率为103．9％，RSD为1．20％；槲皮素平均回收率为100．5％，RSD为0．97％。结论HPLC法测定没食予酸和槲皮素，方法简便可行，重复性和分离效果好。
英文摘要：
Objective To determine the Championi （Benth.） Benth. Methods The separation contents of gallic acid and quercetin in Bauhinia was performed on SinoChrom C18（4.6mm×250mm,5μm）） with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate was 1.0 mL·min^-1, the detective wavelength was 255 nm, and the column temperature was 30℃. Results The linear ranges of gallic acid and quercetin were 0.162-3.24 μg, 0.002052-0.041μg respectively, the average recoveries were 103.9% （RSD= 1.20%）, 100.5% （RSD=0.97%） respectively. Conclusion The method is simple and reproducible, and it has good separation effect.
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