中文摘要：

我们的以前的研究显示 TCP1 是 brassinosteroid (BR ) 的一个积极管理者由调停的生合成小径 DWF4 的抄写，在 Arabidopsis thaliana 的关键 BR biosynthetic 基因之一。然而， TCP1 是否能直接绑在 DWF4 的倡导者区域，试验性地没被表明。这里，我们提供我们 TCP1 调停的生物化学、基因的证据由直接在 DWF4 的倡导者区域与二个 GGNCCC 主题联系的 DWF4 的表示。DWF4 的表示层次断然在 planta 被相关到 TCP1 丰富。用各种各样的合成 DNA 碎片的 Electrophoretic 活动性移动试金(EMSA ) 建议 GGNCCC 核心顺序为 TCP1 绑定是批评的。DNA 序列 flanking GGNCCC 主题为 TCP1 的协会是不太重要的。用是的 DWF4p-GUS 转基因的植物一个试金系统，这些主题被 TCP1 为 DWF4 抄写的积极规定要求，这清楚地被显示。更显著地，整个染色体 microarray 分析显示 TCP1 能直接或间接地调整知道为正常植物生长和开发重要的许多另外的基因的表达式。

英文摘要：

Our previous studies indicated that TCP1 is a positive regulator of the brassinosteroid （BR） biosynthesis pathway by mediating the transcription of DWF4, one of the key BR biosynthetic genes in Arabidopsis thaliana. Whether TCP1 can directly bind to the promoter region of DWF4, however, has not been experimentally demonstrated. Here we provide our biochemical and genetic evidence that TCP1 mediates the expression of DWF4 by directly associating with the two GGNCCC motifs in the promoter region of DWF4. The expression levels of DWF4 are positively correlated to TCP1 abundance in planta. Electrophoretic mobility shift assays （EMSAs） using various synthetic DNA fragments suggest that the GGNCCC core sequence is critical for TCP1 binding. DNA sequences flanking the GGNCCC motifs are less important for the association of TCPI. Using DWF4p-GUS transgenic plants as an assay system, it is clearly indicated that these motifs are required for the positive regulation of DWF4 transcription by TCP1. More significantly, whole genome microarray analyses indicate that TCP1 can directly or indirectly regulate the expression of many other genes known to be important for normal plant growth and development.