采用室内浸叶法,测定了华中地区5个蔬菜产区(河南洛阳白马寺镇、湖南岳阳广兴洲镇、湖北武穴梅川镇、湖北宜昌当阳市、湖北云梦隔蒲镇)田间小菜蛾种群对2%阿维菌素乳油(EC)、16 000 IU·mg-1Bt工程菌WG-001可湿性粉剂(WP)、20%高效氯氰菊酯EC、5%氯虫苯甲酰胺EC、2.5%多杀菌素悬浮剂(SC)、10%溴虫腈EC、5%氟啶脲EC、20%丁醚脲EC、5%茚虫威EC等9种常用杀虫剂的抗药性。结果表明:5个小菜蛾种群对阿维菌素(抗药性倍数为44.5~92.0倍)、Bt(抗药性倍数为66.5~202.1倍)、高效氯氰菊酯(抗药性倍数为76.2~146.4倍)已产生了高水平至极高水平抗性,对氟啶脲(抗药性倍数为32.5~82.5倍)的抗药性达到中等至高水平抗性,对多杀菌素(抗药性倍数为6.5~19.4倍)、氯虫苯甲酰胺(抗药性倍数为3.1~23.1倍)、溴虫腈(抗药性倍数为2.6~22.3倍)和茚虫威(抗药性倍数为7.1~26.3倍)表现为中等及以下水平抗性,对丁醚脲(抗药性倍数为2.3~7.7倍)表现为敏感至低水平抗性。因此,华中地区的小菜蛾防治应注重氯虫苯甲酰胺、溴虫腈、丁醚脲与多杀菌素、茚虫威、氟啶脲交替、轮换使用,以便延缓抗药性的产生与发展,取得更好的防效。
Five field populations of diamondback moth,Plutella xylostella,including Luoyang in Henan Province,Yueyang in Hunan Province,and Wuxue,Yunmeng and Yichang in Hubei Province, were collected from the central China.P. xylostella populations had resistance to 9 insecticides as 2%abamectin EC,16 000 IU·mg-1 Bt WG-001 WP,20%beta-cypermethrin EC,5%chlorantraniliprole EC,2.5% spinosad SC,10% chlorfenapyr EC,5% chlorfluazuron EC,20% diafenthiuron EC,5%indoxacarb EC.The bioassay results indicated that 5 populations exhibited high to extreme high level of resistance to abamectin,Bt,beta-cypermethrin,with the resistance ratio 44.5-92.0 fold,66.5-202.1 fold,and 76.2-146.4 fold,respectively.Moderate to high level of resistance to chlorfluazuron was found with the ratio 32.5-82.5 fold.As to spinosad,chlorantraniliprole,chlorfenapyr,indoxcarb, moderate and low resistance levels were proved with the ratio 6.5-19.4 fold,3.1-23.1 fold,2.6-22.3 fold,and 7.1-26.3 fold.The resistance ratio to diafenthiuron was ranged from 2.3-7.7 fold,showing sensitive or low resistance.Therefore,attention should be paid to insecticides rotation application be-tween chlorantraniliprole,chlorfenapyr,diafenthiuron which were more sensitive and spinosad,in-doxacarb,chlorfluazuron in central China,so as to delay the generation and development of insecticide resistance and achieve better control effect.