中文摘要：

超临界流体色谱（SFC）分离具有速度快、分离效率高、溶剂消耗少等优点,近年来在手性化合物的分离分析中得到诸多应用。本文对比研究了涂覆型多糖手性色谱柱在SFC和高效液相色谱（HPLC）上拆分24种手性化合物的差异。通过比较这些化合物在色谱柱上的保留时间和选择因子等发现多数化合物在SFC上的分离效率要高于其在HPLC上的分离效率,但HPLC对轴手性化合物的分离效率要优于SFC。SFC和HPLC的分离表现出一定的互补性,随着苯环侧链烷基的碳数增加,化合物在SFC上的保留逐渐增强,而在HPLC的保留却逐渐减弱。叶菌唑在使用SFC和HPLC分析时出现了洗脱顺序反转的现象。这些结果为SFC手性拆分提供了参考。

英文摘要：

Supercritical fluid chromatographic（SFC）technique has been widely applied in the analysis,determinations and chromatographic preparations of chiral compounds in past decades due to some advantages such as fast speed,high efficiency and low consumption of organic solvents. Herein,24 kinds of chiral compounds（H1-H24）have been resolved on four polysaccharide-coated chiral stationary phases（CSPs）by SFC and high performance liquid chromatography（HPLC）,separately. Taking into account their retention times and selection factors of these compounds,SFC separation showed a better efficiency than HPLC for most of the compounds,except for some axis-chiral compounds. Some complementarity between SFC and HPLC separations was observed. For H15- H17,their retentions in SFC separations showed a strengthening trend with increasing carbon numbers of side alkyl chain,whereas a reverse tendency in HPLC separation was displayed. Most interestingly,an elution order reversal of metconazole enantiomers was found in this work. In brief,these results would provide some valuable references for the development of SFC separation methods in the future.