中文摘要：

目的：探讨周期性动态压力对人牙周膜干细胞（h PDLSCs）膜片中力生长因子（MGF）表达的影响。方法：取第3代h PDLSCs及其膜片,并按照不同压力将其随机分为0（对照）、0～90、0～120、0～150 KPa组后,置于细胞压力加载室内以0.1 Hz频率连续加载1 h。分别于加压后0、12、24、36 h各时间点,用CCK-8法检测各组细胞的增殖;RT-PCR法检测各组h PDLSCs膜片中MGF、ALP、Col-ⅠmRNA的表达。结果：0～120 KPa组可显著促进h PDLSCs增殖（P〈0.05）,其次为0～150 KPa组;各周期性动态压力作用于h PDLSCs膜片后12 h均能明显上调其MGF mRNA表达水平（P〈0.05）,受力后24 h达到峰值;36 h后表达水平下降,除0～120 KPa组仍明显高于对照组（P〈0.05）外,其他两组均回到对照组水平。其中以0～120 KPa组的MGF mRNA表达水平最高（P〈0.05）,且在该压力条件下还能明显促进h PDLSCs膜片中Col-Ⅰ、ALP mRNA的表达（P〈0.05）。结论：0～120 Kpa/1 h周期性动态压力能促进h PDLSCs膜片中MGF mRNA的表达,促进该细胞的增殖与分化潜能。

英文摘要：

AIM： To investigate the effects of periodic cyclic hydrostatic pressure on the expression of mechano-growth factor（ MGF） in human periodontal ligament stem cell（ h PDLSC） sheets. METHODS： h PDLSC sheets were prepared passage 3 h PDLSC sheets were treated by cyclic hydrostatic pressure at 0 KPa（ control）,0- 90 KPa,0- 120 KPa and 0- 150 KPa for 1 h / 0. 1 Hz,respectively. At different time points（ 0,12,24 and 36 h respectively）,cell proliferation rate was measured by CCK-8,the mRNA expression of MGF,ALP and Col- Ⅰwas examined by real-time PCR. RESULTS： The pressure of 0- 120 KPa promoted cell proliferation（ P 〈 0. 05）. Hydrostatic pressure stimulation caused a quick increase of MGF mRNA at 12 h,MGF mRNA reached peak at 24 h and returned to normal control level at 36 h. 0- 120 KPa group showed the strongest stimulating effect on MGF mRNA expression.Furthermore,it also increased ALP and Col- I expression（ P 〈 0. 05）. CONCLUSION： Cyclic hydrostatic pressure at 0- 120 KPa /1 h may promote the expression of MGF and increase the proliferation and differentiation of h PDLSCs.