中文摘要：

目的内源性前列腺素（prostaglandin,PG）D2能增加基底前脑下蛛网膜下腔内的腺苷水平,可能作用于腺苷A2A受体,诱发睡眠。本实验观察腺苷A2A受体特异性激动剂CGS21680和PGD2促眠作用的异同。方法手术插入不锈钢导管至大鼠基底前脑下蛛网膜下腔,同时埋植脑电波和肌电波记录电极,术后2周,经导管微量恒速灌注PGD2（200pmol·0.2μL^-1·min^-1）或CGS21680（10pmol·0.2μL^-1·min^-1）,同步记录脑电波和肌电波,分析睡眠量、睡眠强度及睡眠时相转换等参数。结果与人工脑脊液自身对照相比,PGD2和CGS21680都显著增加慢波睡眠（非快动眼睡眠）量和睡眠强度,两者在时相转化和各波段时程上表现出高度的相似性。CGS21680增加快动眼睡眠量,而PGD2不引起快动眼睡眠量的增加。结论腺苷A2A受体激动剂CGS21680能模拟PGD2诱发的慢波睡眠,提示腺苷A2A受体是诱导生理性睡眠的重要靶点之一。

英文摘要：

OBJECTIVE To compare the sleep-wake profiles induced by endogenous prostaglandin （PG）D2 and CGS21680,an adenosine A2A receptor agonist. METHODS Under 10% chloral hydrate anesthesia [360 mg·kg^-1 intraperitoneal （i.p.）],rats underwent the surgery for implantation of electrodes for electroencephalogram （EEG） and electromyogram recordings and placement of a stainless steel cannula inserted stereotaxically into the subarachnoid space under the basal forebrain for infusion of PGD2 or CGS21680. The software SleepSign was used to analyze EEG parameters,including sleep amount,sleep episode,stage transition and power density. RESULTS PGD2 and CGS21680 induced non-rapid eye movement sleep was similar to physiological sleep with the same amounts,identical patterns of sleep episode number and duration,stage transition,and power density. CONCLUSION Activation of adenosine A2A receptors by CGS21680 mimicked the somnogenic effects of PGD2.