中文摘要：

生物大分子定点标记的荧光探针可以用来研究蛋白质的结构和功能,荧光探针良好的刚性和高连接特异性对于使用荧光共振能量转移（FRET）实验来解析生物大分子动态学特征来说有着重要的意义。本文报道两种花菁素类荧光探针IAM-Cyanine3和IAM-Cyanine5的合成方法,该探针通过碘乙酰胺基团特异性地标记在生物大分子的巯基上,相对于商业化的产品,其连接蛋白后的探针分布更加紧密,更有利于对生物大分子的结构和动态学进行更加精确的描述。

英文摘要：

The fluorescence probe for specifically bio-molecule site labeling could be used to investigate structures and functions of proteins. The rigidity and high labeling selectivity of the fluorescence probe is critical to the study on protein dynamics using FRET. Here,we describe two synthesis methods of two cyanine probes,IAMCyanine3 and IAM-Cyanine5. The two probes are attached onto the protein surface through reaction between the iodacetamide group of the probe and the thiol group on the protein surface. Compared to common commercial probes,our probes possess lower flexibility after being attached onto proteins, leading to better resolution of biomacromolecule dynamics and structures.