中文摘要：

目的探讨吡格列酮（Pio）调节树突状细胞（DC）黏附和迁移功能的可能机制。方法体外培养人外周血单核细胞来源的DC和人脐静脉内皮细胞，使用不同浓度的Pio及加过氧化物酶体增殖物活化受体-1拮抗剂（GW9662）干预DC24h，Western印迹和免疫荧光试验检测Pio对表达DC特异性细胞间黏附分子3捕获的非整合蛋白（DC-SIGN）的影响，细胞黏附试验检测Pio对DC黏附功能的作用，细胞迁移试验检测Pio对DC迁移功能的作用。结果（1）Pio呈浓度依赖性下调DC．SIGN蛋白的表达，在Pio1．0μmol／L时Dc-SIGN蛋白表达量明显减少（0．96±0．09，对照1．25±0．23，均P〈0．05），Pio 10μmol／L时减少更明显（0．80±0．08，均P〈0．01），GW9662干预组（1．10±0．12，均P〈0．01）蛋白表达量增加；（2）在Pio 1．0μmol／L时DC黏附和迁移降低（10．8％±2．0％和24．6％±0．5％，均P〈0．05），Pio 10μmol／L时降低更明显（7．6％±1．5％和23．4％±3．0％，均P〈0．01），GW9662干预组DC黏附和迁移增加（12．1％±1．9％和26．8％±0．8％），经抗DC—SIGN抗体作用后DC黏附和迁移较对照降低（5．7％±0．9％和23．2％±2．9％，均P〈0．01）。结论Pio能通过激活过氧化物酶体增殖物活化受体-γ，下调DC-SIGN蛋白表达，从而抑制DC的黏附和迁移功能。

英文摘要：

Objective To investigate the effect of piogiitazone （Pio） on dendritic cell-（DC） specific intercellular adhesion molecule-3 grabbing nonintegrin （DC-SIGN） expression in DCs and explore the possible mechanism of Pio inhibiting DC adhesion and transmigration. Methods DCs derived from human peripheral blood mononuclear cells were cultured and divided into 6 groups： blank control group, Pio 0.1 μmol/L group, Pio 1.0 μmol/L group, Pio 10 μmol/L group, GW9662, a peroxisome proliferator activated receptor （PPAR）-γ antagonist, 10 μmol/L group, and GW9662 10 μmol/L ＋ Pio 10μmol/L group. Western blotting was used to detect the protein expression of DC-SIGN 24 h later. Human umbilical vein endothelial cells （HUVECs） were obtained and co-cultured with the DCs undergoing different treatments. Immunofluorescence test was used to detect the protein expression of DC-SIGN. DCs labeled with 5-chloromethylfluorescein diacetate （CMFDA） were added into the monocellular layer of fused ECs. Blank DCs and DCs pretreated with anti-DC-SIGN antibody were used as blank and experimental groups. Laser confocal microscopy was used to observe the adhesion ability of the DCs. HUVECs were inoculated into the upper chamber of Transwell plate and CMFDA-labeled DCs of above mentioned groups were added to the mono-cellular layer of these ECs. Serum-free culture medium with monocyte chemoattractant protein-1 was added into the lower chamber. Eight hours later the transmigration ability was observed. Results Western blotting showed that the DC-SIGN protein expression levels of the DCs of the Pio 1.0 μmol/L and Pio 10 μmol/L group were 0.96 ± 0.09 and 0.80 ± 0.08 respectively, both significantly lower than that of the blank control group （ 1.25 ± 0.23, P 〈 0.05 and P 〈 0.01 ） ; and the DC-SIGN protein expression level of the GW9662 10 μmol/L ＋ Pio 10 μmol/L group was 1.10 ± 0.12, significantly higher than that of the Pio 10 μmol/L group （P 〈 0.05）. Immunofluorescence test showed that the DC-SIG