中文摘要：

目的：探讨异基因骨髓源间充质干细胞（Bonemarrow derived mesenchymal stem cells，BMSCs）对BXSB小鼠T、B细胞增殖、活化及功能成熟等方面的影响。方法：采用。H．TdR掺入法、FACS法、EIJSA等方法检测BMSCs对BXSB小鼠T、B细胞的影响。结果：BALB／c小鼠的BMSCs在不影响BXSB小鼠T细胞诱导活化的基础上抑制其诱导增殖；可降低由ConA诱导的CD4^＋IL^＋细胞的数量，而提高由ConA诱导的CD4^＋IFN-γ^＋细胞数量。对于B细胞，BALB／c小鼠的BMSCs可以抑制其增殖、活化及IgG的分泌。另外，BALB／c小鼠的BMSCs可以抑制BXSB小鼠B细胞上CIMOL的异位表达。结论：异基因BMSCs对自身免疫病小鼠的T、B细胞有一定的调节作用。

英文摘要：

Objective：To investigate the immunoregulatory effects of allogeneic BMSCs on T and B cells from BXSB mice with SLE. Methods： In this work,^3H-TdR incorporation assay, ELISA, FACS were performed to evaluate the effects of BMSCs on immunocompetent cells of BXSB mice. Results： BMSCs from BALB/c had the inhibitory effects on BXSB mice T lymphocytes proliferation without affecting their activation;Significant inhibition on ConA-induced CD4^＋ IL-4^＋ producing cells and enhancing effects on CD4^＋ IFN-γ^＋ producing cells;As to B lymphocytes of BXSB mice, BMSCs had inhibitory effects on their proliferation, activation and IgG secretion; In addition, BALB/c BMSCs had inhibitory effects on CD40 hgand （CD40L） ectopic hyper-expression on B cells of BXSB mice. Conclusion： Allogeneic BMSCs had some immunoregulatory effects on T and B cells of BXSB mice with SLE.