ftsz基因是控制细胞分裂的关键基因,其蛋白能够在分裂位点形成一个环状结构而影响细胞分裂。为了研究木薯质体分裂与木薯淀粉品质形成的关系,根据木薯基因组数据库上的预测序列,设计引物,从木薯基因组中分离了与质体分裂相关的.胚z家族3个新基因(ftsz1,ftsz2,ftsz3)。分别将它们与荧光蛋白基因(GFP)融合,构建了3个原核表达载体pET-fisZl-GFP、pET-胚忍-GFP、pET-fisZ3-G即,并转化大肠杆菌BL21(DE3)。通过荧光显微镜观察菌体的表型和分裂,初步鉴定了木薯质体分裂相关基因.胚z家族对细胞分裂的作用。结果显示:尽管木薯与大肠杆菌的亲缘关系较远。ftsz基因的同源性较低,但是两者表现出相似的功能,木薯ftsz基因的表达能严重影响大肠杆菌细胞分裂。这一结果为进一步研究木薯ftsZ家族基因的功能奠定了基础。
The fisZ genes are a set of key genes in cell division regulation, and their proteins form a ring structure at the division site to control the process of cell division. In order to study the relationship between cassava starch formation and amyloplast division, three cassava fisZ family genes were isolated and named as fisZ1-3. To identify their functions, the fisZl-3 genes respectively fused with GFP were expressed in E. coli BI221 (DE3). The result showed that the expression of cassava fisZ genes actively affect the E. coli division although the genetic relationship between cassava and E. coli is far, and the homology of theirfisZ genes is low. This result will be a foundation for further study the function of cassava fisZ family genes.