中文摘要：

以AlsCBF基因cDNA和植物表达载体pCAMBIAl301进行双酶切，将抗寒基因AlsCBF与表达载体正向选择系统结合，构建抗寒基因植物表达载体pCAl301-AlsCBF。对阳性克隆通过菌落PCR、酶切鉴定和测序分析，成功将目的基因序列正确连接到pCAMBIAl301，构建其植物表达载体，并转入根癌农杆菌LBA4404中。

英文摘要：

This study was carried out to cut double enzyme withAlsCBF gene cDNA and plant expression vectors pCAMBIA1301, to combine cold-resistant gene AlsCBF with positive selection system to construct plant expression vectors of cold-resistant gene. In positive cloning, through the colonies PCR, enzymatic di- gestion and sequencing analysis, the purpose gene sequence will be properly connected with the pCAM- BIA1301 to build the plant expression vectors and to be moved on into Agrobacterium tumefaciens LBA4404.