中文摘要：

利用原子力显微镜观察了牛胰岛素（bovineinsulin）在高湿度环境下（相对湿度大于90％）、在4种不同衬底表面上的聚集行为．在高湿度环境下，衬底表面会形成一层纳米级水膜，牛胰岛素分子会在水膜中扩散、迁移、聚集．原子力显微镜观察表明，牛胰岛素在不同衬底表面呈现出形式多样的聚集状态：在MICA表面形成排列比较规则的纤维；在APS—MICA表面呈现出较杂乱的聚集状态；在高温热解石墨（HOPG）表面蛋白分子主要吸附在HOPG的台阶上，堆积成高点并沿着台阶呈线状排列；在OTS-MICA表面，部分区域出现团聚，小部分聚集成纤维状．这些结果表明：在高湿度条件下，不同衬底表面对牛胰岛素的聚集有很大影响，可以利用不同性质的衬底表面来调制牛胰岛素的聚集．

英文摘要：

The assembly of bovine insulin on four different substrates under high humidity environment was investigated by atomic force microscopy. Bovine insulin could diffuse and coalesce on surface due to a thin water layer covered on the surface in a high humidity environment. The aggregation behavior of insulin was highly modified by different surfaces. A highly ordered trigonal arrangement was displayed on MICA surface. Short and kinkled fibrils were formed on APS-MICA. On HOPG, insulin stayed along the steps and formed a little big aggregation. While on OTS-MICA surface, some big aggregations and ordered trigonal arrangements were observed. These results indicate that the properties of surface have a large influence on the assembly of bovine insulin under high humidity, and thus the assembly of fibrils may be controlled using a substrate with particular surface properties.