中文摘要：

为进一步了解抗菌肽的研究现状,以期为抗菌肽的纯化鉴定、生产和应用提供理论依据,对抗菌肽的功能,纯化鉴定方法,人工合成和基因工程表达进行了综述。抗菌肽不仅具有广谱的抗菌活性且有抗癌、抗病毒、促进伤口愈合等作用,抗菌肽经乙酸提取,反相液相色谱纯化是较常见的方法,抗菌肽的人工合成多采用固相合成,基因重组一般包括三个内容：抗菌肽的基因结构分析,抗菌肽的基因表达调控及互补脱氧核糖核酸（cDNA）编码,抗菌肽的基因表达系统。

英文摘要：

In order to further understand the research progress of antimicrobial peptides, and provide theoretical basis for the purification, identification, production and application of antimicrobial peptides, the function and method of purification, identification, synthetic and genetic engineering of the antimicrobial peptide were summarized. The antibacterial peptides not only have a broad spectrum antimicrobial activity, but also have the effects of antitumor, antiviral, promoting wound healing, etc. The common treatment methods for antibacterial peptides were acetic acid extraction and reversed phase liquid chromatography purification. The method for antibacterial peptide synthesis was solid phase synthesis. Genetic recombination generally includes three contents： genetic structure analysis of antibacterial peptides, regulation of antibacterial peptide gene expression, c DNA encoding and antibacterial peptide gene expression system.