中文摘要：

目的：观察并探讨＂通督启神＂法电针治疗对阿尔茨海默病（AD）小鼠学习记忆能力和大脑皮层葡萄糖代谢水平的影响。方法：将7月龄APP/PS1双转基因小鼠20只,随机分为AD模型组和＂通督启神＂法电针组,以同背景同月龄C57BL/6小鼠10只作为正常对照组。＂通督启神＂法电针组给予电针百会、印堂穴20min,随后点刺人中穴;正常对照组和AD模型组给予＂通督启神＂法电针组相同程度和时间的束缚。连续治疗15d后,采用Morris水迷宫观察各组小鼠空间学习记忆能力的变化,应用Micro-PET显像技术定量分析各组小鼠大脑皮层葡萄糖代谢水平的变化。结果：与AD模型组比较,正常对照组与＂通督启神＂法电针组在隐蔽平台试验中逃避潜伏期均显著性下降,在空间探索试验中目标象限游泳路程占比均明显增加（P〈0.05）。Micro-PET显像结果表明,AD模型组小鼠额叶、颞叶和顶叶皮层18F-FDG摄取率低于正常对照组（P〈0.05）,＂通督启神＂法电针组高于AD模型组（P〈0.05）。结论：＂通督启神＂法电针治疗可一定程度改善AD小鼠学习记忆能力,提高大脑葡萄糖代谢水平。

英文摘要：

Objective： To observe the influence of electro-acupuncture therapy of ‘Tongdu Qishen＇ on learning and memory capacity and cortical glucose metabolism of mice with Alzheimer＇s disease（AD） by Morris water maze combined with micro-PET imaging. Methods： Twenty seven-month-old APP/PS1 mice were randomly divided into AD model group（M） and electro-acupuncture group（EA）, 10 seven-month-old C57BL/6 mice were used as normal control group（N）. Electro-acupuncture was applied to Baihui（GV20） and Yintang（GV29） for 20 min per day and then swift pricking blood therapy was given at Shuigou（GV26）. Mice in N and M groups were received restriction for 20 min. After 15 days treatment, Morris water maze was performed to observe behavioral changes of mice, and the cortical glucose metabolism quantitative analysis were detected by positron emission tomography imaging. Results： Compared with M group, the escape latency of N and EA groups were declined significantly, while the proportion of the platform quadrant swimming distance in total swimming distance were obviously increased（P〈0.05）. The Micro-PET imaging showed mice in M group performed a lower of glucose in cortex compared to N group（P〈0.05）, and EA group presented a higher injected dose while compared with M and N groups（P〈0.05）. Conclusion： To a certain extent, electro-acupuncture therapy of ‘Tongdu Qishen＇ might improve the learning and memory capacity and increase glucose metabolism in cortex of AD mice.