长棘海星(Acanthaster planci)是热带和亚热带海域珊瑚礁的主要破坏者,体内外有丰富的共附生微生物。该文对采自南海三亚珊瑚礁保护区的一个长棘海星样品的共附生真菌,利用3种培养基进行分离纯化得到近200株真菌菌株。采用通用引物 ITS1和 ITS4扩增其中16株真菌的 ITS-rDNA 片段序列,并进行测序,测试结果提交 GenBank,登录号为 KF999808-KF999822以及 KJ723458。利用邻接法构建系统发育树及简单分析该长棘海星共附生真菌的种群多样性。序列分析显示,16株真菌分别属于 Ascomycota 门4目(Hypocreales、Eurotiales、Pleosporales、Microascales),5属(Trichoderma、Penicillium、Neosartorya、Leptosphaerulina、Pseudallescheria),以及 Deuteromycota 门1目(Meliolales),1属(Fusarium,有性型 Gibberella),并聚为8类。多样性分析的结果显示,菌体的个体数在镰刀属上(10株)表现为高度富集,群落的均匀度比较低。
Acanthaster planci,commonly known as the crown of thorns starfish,usually preys upon scler-actinian corals.Recently,Acanthaster planci has been found biocenotic association with diverse and a-bundant microbial communities.Using three different media,nearly 200 strains of the endophytic fungi were isolated from the Acantaster planci collected from the Sanya,Hainan province,China.The ITS-rD-NA gene of 16 strains were sequenced,and submitted to GenBank.The register numbers of the se-quences for these strains are KF999808-KF999822 and KJ723458.The phylogenetic tree was then con-structed by using a neighbor-joining method,as well as the population diversity analysis.16 strains be-longing to 2 phyla (Deuteromycota,Ascomycota),5 orders (Hypocreales,Eurotiales,Meliolales,Mi-croascales),6 genera (Trichoderma,Pseudallescheria,Penicillium,Neosartorya,Leptosphaerulina, Pleosporale,Fusarium,)were classified into 8 groups.And ten strains belonging to Fusarium indicates that Fusarium is the dominant fungi,and the endophytic fungi community with form of commensalism of Acanthaster planci has the low degree of homogeneity.