中文摘要：

利用具有猝灭植物青枯菌（Ralstonia solanacearum）群体感应信号分子功能的aac基因构建高效植物表达载体,通过根癌农杆菌（Agrobacterium tumefaciens）介导的方法转化烟草（Nicotiana tabacum）,获得了卡那霉素抗性植株57株。经PCR及RT-PCR检测,筛选得到30株阳性转基因再生苗,并证实了目的基因已经整合到烟草基因组中,且在转录水平上得到表达。转基因植株苗期抗青枯病试验表明,16d后转基因烟草较对照植株病情指数降低了51,相对病情指数为56.5%。

英文摘要：

Plant expression vector pBI121/Ω4A/aac was constructed by cloning of aac gene which could quench the quorum-sensing of Ralstonia solanacearum. The aac gene was transferred into tobacco （Nicotiana tabacum） byAgrobactenummediated. Under kanamycin selection pressure, 57 resistant lines were obtained. PCR and RT-PCR analysis indicated that aac gene had been integrated into tobacco genome and translated, Inoculation test with the Bacteria wilt showed that the disease index of transgenic tobacco was reduced by 51 in greenhouse after 16 d of inoculation comparing with the control, and the Relative Disease Index was 56.5%.