中文摘要：

生物固氮是海洋氮循环的重要过程。固氮作用能够促进海洋初级生产力的提高，增强海洋吸收CO2能力，对于降低大气CO2浓度，减缓温室效应具有重要意义。2007年春季对北部湾海区进行了现场模拟添加P，Fe和胶体实验，并应用乙炔还原法（ARA，Acetylene Reducing Activity）分析其固氮速率，研究其主要影响因素。结果表明，温盐与固氮速率相关性不显著（P 〉 0.05）。对固氮速率来说，外加P其变化范围为-97%-545%，外加Fe其变化范围为-86%-146%，外加胶体其变化范围为-96%-1456%。对叶绿素含量而言，外加P其变化范围为-24%-50%，外加Fe其变化范围为-32%-36%，外加胶体其变化范围为-53%-41%。N可能是春季北部湾海区浮游植物生长的限制因子，固氮作用主要受到P限制，而外加胶体对固氮生物和固氮速率表现出较大的促进作用。

英文摘要：

Nitrogen fixation is an important process in marine nitrogen recycling. It can enhance marine primary productivity and marine carbon fixation capacity and reduce CO2 concentration in the atmosphere, thus mitigating the greenhouse effect. The Beibu Gulf is a semi-enclosed bay located northwest of the South China Sea. In the Beibu Gulf, P and Fe are influenced by river input and atmospheric deposition, and the water in Beibu Gulf is rich in colloids. In order to study the effects of P, Fe, and colloids on the nitrogen fixation rate and nitrogen fixation in response to the nutritional environment, we conducted a field addition experiment for P, Fe, and colloid simulation in the spring of 2007. Surface water samples were collected from nine study sites between April and May 2007. P, Fe, and colloids were separately added for a scene simulation in the study sites of C02, C05, C07, and C09. Concentration gradients of P （potassium dihydrogen phosphate） were set as C, C＋0.08, C＋0.16, C＋0.32, and C＋0.48 μmol/L. Concentration gradients of Fe were set as C, C＋0.22, C＋0.44, C＋0.88, and C＋1.32 μmol/L （C is the concentration of nutrients in in situ water samples）. Concentration gradients of colloids （particle size, between 0.22 μm and 10 kDa） were set as 0%, 2%, 50%, and 100%. The acetylene-reducing activity method was used to study nitrogen fixation and factors that affect it. The results showed that the contributions of temperature and salinity to the nitrogen fixation rate were low, while P, Fe, and colloids increased the nitrogen fixation rate. The nitrogen fixation rate was greatly promoted by adding colloids, while the addition of P and Fe did not affect the nitrogen fixation rate to a great extent. However, variations in the nitrogen fixation rate due to the addition of P, Fe, and colloids were -97% to 545%, -86% to 146%, and -96% to 1456%, respectively, and variations in chlorophyll contents were -24% to 50%, -32% to 36%, -53% to 41%, respectively. Differences in the nitrogen fixation rat