基于1-萘酚、2-萘酚荧光光谱扫描时激发波长与发射波长所存在的差异,建立了一种利用荧光光度法同时检测痕量的1-萘酚、2-萘酚的方法。文章综合考虑了pH值、温度、表面活性剂等因素对目标物检测的影响,同时研究了类似有机物和无机离子对本方法的干扰情况。与传统方法相比,该方法无需采用复杂的预处理步骤,无需昂贵的仪器设备,方法简单、快速,精密度和准确度高,对1-萘酚、2-萘酚的相对标准偏差(RSD)分别为1.26%和1.45%;检出限均为8.6×1 0-3ng.mL-1;该法用于合成样品和模拟的环境水样中1-萘酚、2-萘酚的同时测定,当1-萘酚2-萘酚比在1∶19~19∶1之间时,实验结果满意,回收率在95%~105%之间。说明本方法简单,灵敏,结果可靠,可用于环境水样中1-萘酚,2-萘酚的同时检测。
A simple and sensitive method for the simultaneous determination of trace amounts of 1-naphthol and 2-naphthol by fluorescence spectrofluorimetry was established based on the fact that the excitation and emission wavelengths are different between 1-naphthol and 2-naphthol.The influencing factors,such as pH temperature and surface activator were synthetically considered in the present paper.Interference of similar organic matters and inorganic ions were also taken into account.Compared with traditional methods,the method discussed here needs no complex pretreatments and expensive equipment.So it is convenient,quick and cheap yet with a satisfactory precision and accuracy.The accuracy and precision of results obtained are satisfactory when the concentration ratio(CR) of 1-naphthol to 2-naphthol is in the range of 1:19-19:1,the detection limit(DL) of both naphthols is 8.6×1 0-3 ng·mL-1,the relative standard deviation(RSD) of 1-naphthol and 2-naphthol is 1.26% and 1.45% respectively,and the recovery ratio(RR) of both naphthols is between 95% and 105%.This proposed method has been applied to the determination of trace amounts of 1naphthol and 2-naphthol in the man-made sample water and environmental imitative water simultaneously,and obtained a satisfactory result,showing that the method discussed here is simple,sensitive and reliable in the future use.