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中文摘要：
目的了解Ⅰ类、Ⅱ类和Ⅲ类整合子在临床分离铜绿假单胞菌中的分布,探讨整合子携带与耐药关系。方法菌株的鉴定采用API板条,药物敏感性测定采用K-B纸片扩散方法,整合子检测采用PCR方法。结果本院临床分离的60株铜绿假单胞菌中,21株（35%）Ⅰ类整合子检测阳性,2株（3.3%）Ⅱ类整合子检测阳性,未检出Ⅲ类整合子;对于所测试的抗菌药物除亚胺培南和多黏菌素外,Ⅰ类整合子阳性菌株的耐药率明显高于Ⅰ类整合子阴性菌株（P〈0.05）。结论本地区临床分离的铜绿假单胞菌携带的整合子类型为Ⅰ、Ⅱ类整合子,且Ⅰ类整合子与铜绿假单胞菌耐药密切相关。
英文摘要：
Objective To investigate the prevalence of type Ⅰ,Ⅱ and Ⅲintegrons in clinical isolates of Pseudomonas aeruginosa and to analyze the relation between the presence of integrons and antibiotic resistance.Methods All the clinical bacteria isolates were identified by API system,antimicrobial resistance was determined by K-B disk diffusion methods and screening of integrons was performed by PCR.Results Among the 60 clinical isolates of Pseudomonas aeruginosa,21 isolates（35%） carried type Ⅰ integron,2 isolates（3.3%） carried type Ⅱ integron,and no isolate carried type Ⅲ integron.The resistance rates to the antibiotics tested were significantly higher in integronⅠ-positive isolates than negative ones（P＆lt;0.05）,except for Polymyxin and Imipenem.Conclusion Type Ⅰ and type Ⅱ integrons might be the prevalent integron types in the region and the presence of type Ⅰ integrons was closely linked to antibiotic resistance.
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