中文摘要：

传统的五分类血液分析仪一般与化学方法配合使用以提高白细胞的分类精度,增加了仪器结构的复杂程度和仪器的使用、维修成本。设计了一种特殊结构的光学系统,该系统引入了对后向散射光的检测,并采用直流（DC）信号、激光前向散射信号和后向散射信号相结合的方式实现了白细胞五分类。实验结果表明装有该系统的样机对LYM、MON、NEU、EOS、BAS的分类相对偏差均小于5%,其性能与血液分析仪Mythic 22接近。优化后的光学系统可完成对白细胞的原位检测和分类,且具有结构简单、性能稳定的特点。

英文摘要：

Traditional five classification hematology analyzers usually improve white blood cell（WBC） classification accuracy by combining chemical methods, which will increase the complexity of instrument structure and the cost of use and repair. An optical system with a special structure is designed, which introduces the detection of back scattered light by the means of combining direct current（DC） signal, laser forward scattering signal and back scattering signal to achieve the WBC five classification. The experiment results show that the relative deviation of the prototype with the sensing system for LYM, MON, NEU, EOS and BAS is less than 5%, and its performance is close to the hematology analyzer Mythic 22. The optical system after optimization can complete in situ detection and classification of WBC and has the characteristics of simple structure and stable performance.