中文摘要：

运用同源克隆和RACE方法获得了半滑舌鳎（Cynoglossus semilaevis）孕酮受体膜组分I（PGRMCl）的cDNA全长序列，生物信息学分析显示，半滑舌鳎PGRMC1 cDNA序列全长1335bp，开放阅读框长546bp；其编码的蛋白是单次跨膜蛋白，在N端13～35位氨基酸残基处有一个跨膜区域。半滑舌鳎PGRMC1氨基酸序列与青鳝（Oryzias latipes）相似性最高，达到了82．9％；与青斑河纯（Tetraodon nigroviridis）相似度为81．2％。系统进化分析表明，半滑舌鳎PGRMC1与鲔形目和纯形目鱼类PGRMC1聚为一个分支。采用实时荧光定量RT-PCR技术研究发现，性成熟雌性半滑舌鳎PGRMC1 mRNA在各组织广泛表达，其中在卵巢组织中相对表达量最高（P〈0．05）。在不同卵巢发育时期，半滑舌鳎PGRMC1 mRNA在脑、垂体和卵巢中的周期表达变化特征显示：脑中PGRMC1 mRNA在卵巢Ⅲ期表达量升高明显，Ⅴ期表达水平最高（P〈0．05）；垂体中PGRMC1mRNA表达量在卵巢发育V期达峰值（P〈0．05）；在卵巢中，PGRMC1mRNA表达水平从卵巢发育Ⅱ到Ⅴ期稳步上升，Ⅴ期时达到最高值（P〈0．05）。综上，半滑舌鳎PGRMC1基因主要参与卵巢的发育和成熟过程，并在繁殖期介导孕酮调控卵母细胞的最终成熟，研究结果为半滑舌鳎繁殖内分泌调控研究提供了基础资料。

英文摘要：

To investigate the role of progesterone receptor membrane component 1 （PGRMC1） in oocyte maturation in the half-smooth tongue sole （Cynoglossus semilaevis）, we cloned the full-length cDNA sequence of PGRMC1 from the ovary using homology cloning and rapid amplification of eDNA ends methods. The full-length cDNA of PGRMC1 was 1335 bp long with an open reading frame of 546 bp encoding a 181 amino acid prepro- hormone with a deduced molecular mass of 20.64 kDa and a theoretical isoelectric point of 4.67. The precursor was a single transmembrane protein with an N-terminal transmembrane domain. The predicted transmembrane domain was located at positions 13-35 of the deduced PGRMC 1 protein. Sequence alignment of the C. semilaevis PGRMC1 precursor protein with corresponding sequences from other species revealed that the highest identity （82.87%） was with the PGRMC1 from Oryzias latipes, followed by that from Tetraodon nigroviridis （81.22%）. In a phylogenetic analysis, the C. semilaevis PGRMC1 clustered with its counterparts in the Cyprinodontiformes and Tetraodontiformes. The transcript levels of PGRMC1 were high in the ovary, moderate in the liver and brain, and low in other tissues. We also monitored changes in PGRMC1 transcript levels in the pituitary, brain, and ovary at different ovarian developmental stages. The PGRMC1 mRNA levels increased sharply in the brain at stage III, remained at high levels until stage V, and then decreased at stage VI. In the pituitary, the transcript levels of PGRMC1 increased to peak at stage V and decreased significantly after ovulation （stage VI）. Similarly, in the ovary, the PGRMC1 levels increased gradually to peak at stage V and then decreased markedly after spawning （stage VI）. Taken together, our results shed light on the role of PGRMC 1 in oocyte maturation and in regulating reproductive endocrine function in C. semilaevis.