中文摘要：

目的：探讨滋养细胞体外缺氧不同处理方法模拟子痫前期模型的优化研究。方法：HTR8/SVneo滋养细胞分别在1%O2（缺氧环境）和21%O2（常氧环境）培养箱中培养1 h（记为H1、N1）或2 h（记为H2、N2）,缺氧1 h后复氧1 h（记为H1R1）和缺氧2 h后复氧2 h（记为H2R2）,H1R1后再缺氧1 h（记为H1R1H1）,H2R2后再缺氧2 h（记为H2R2H2）,H1R12个循环（记为H1R1H1R1）和H2R22个循环（记为H2R2H2R2）,缺氧2 h后复氧6 h（记为H2R6）,持续缺氧24 h,以及持续常氧4 h（记为N4）、8 h（记为N8）和24 h（记为Normoxia）,然后提取蛋白以Western blot检测腺苷酸活化蛋白激酶（AMP-activated protein kinase,AMPK）磷酸化水平,以验证各组处理对于诱导滋养细胞能量应激的效果;HTR8/SVneo滋养细胞在常氧或者缺氧环境培养24 h后经气相质谱技术（gas chromatography mass spectrum,GS-MS）进行细胞代谢组学分析,以验证AMPK磷酸化水平上调是否与滋养细胞代谢组变化具有一致性。结果：缺氧组AMPK的活性（1.615±0.111）明显高于常氧组（1.000±0.107）和缺氧复氧组（1.277±0.113）;缺氧时显著增加的代谢物有4-甲基-2-戊酮酸（log22.597为1.377）、水合乙醛酸（log22.483为1.312）、组氨酸（log21.188为0.248）、苯丙氨酸（log21.262为0.335）、缬氨酸（log21.518为0.602）、正亮氨酸（log21.519为0.603）、乙酰丝氨酸（log21.691为0.758）、丝氨酸（log21.783为0.834）、半胱氨酸（log21.851为0.889）、蛋氨酸（log22.072为1.051）、鸟氨酸（log22.251为1.170）等;而棕榈反油酸（log20.127为-2.983）、11,14,17-廿碳三烯酸（log20.334为-1.583）、二十四单烯酸（log20.600为-0.738）、共轭亚油酸（log20.680为-0.557）、顺式十八碳烯酸（log20.711为-0.492）、9-十七碳烯酸（log20.782为-0.355）、二十二碳六烯酸（log20.829为-0.271）、二十碳五烯酸（log20.841为-0.250）、芥酸（log20.844为-0.244）、二十二碳五?

英文摘要：

Objective：To investigate the optimal in vitro hypoxia cell mode for preeclampsia study. Methods：Western blot was used to determine the phosphorylation of AMPK after HTR8/SVneo trophoblast cell being treated with 1% O2（hypoxia condition） or21% O2（normoxia condition）for various duration. GC-MS was used for assessing metabolome of HTR8/SVneo trophoblast cell after being incubated in normoxia or hypoxia environment,to validate the synchronous alteration in AMPK phosphorylation and metabolome. Results：The activity of AMPK was higher in hypoxia group（1.615 ±0.111） than in normoxia group（1.000 ±0.107） and hypoxia/reoxygenation group（1.277 ±0.113）. The in-creased metabonomics of the HTR8/SVneo trophoblast cell induced by hypoxia were 4-methyl-2-oxopentanoic acid（log22.597 =1.377）,glyoxylic acid（log22.483=1.312）,histidine（log21.188 =0.248）,phenylalanine（log21.262 =0.335）,valine（log21.518 =0.602）,norleucine（log21.519=0.603）,O-aacetylserine（log21.691=0.758）,serine（log21.783=0.834）,cysteine（log21.851=0.889）,methionine（log22. 072 = 1. 051）,ornithine（log22. 251 = 1. 170）. But palmitelaidic acid（log20. 127 =-2. 983）,11,14,17-eicosatrienoic acid（log20.334=-1.583）,nervonic acid（log20.600=-0.738）,conjugated linoleic acid（log20.680=-0.557）,cis-vaccenic acid（log20.711=-0.492）,9-heptadecenoic acid（log20.782=-0.355）,DHA（log20.829=-0.271）,EPA（log2 0.841=-0.250）,erucic acid（log20.844=-0.244）,DPA（log20.898=-0.156）,citric acid（log20.279=-1.842）,malic acid（log20.208=-2.264）,succinic acid（log20.254=-1.980）,cis-aconitic acid（log20.260=-1.946）,beta-citryl-L-glutamic acid（log20.093=-3.430）,beta-alanine（log20.139=-2.851）,cystathionine（log20.267 =-1.904）,cis-4-hydroxyproline（log20.500 =-1.000） were decreased in hypoxia group. The metabolic pathway analysis shown the nucleotide metabolism [log2（1.811、1.149）=0.857,0.201],the energy metabolism[log2（1.510、1.173、1.149）=0.595,0.230,0.201],the me