叶形状是为在米饭繁殖的 ideotype 的一个重要参数,并且滚动叶对谷物的有效成熟也有益。这鼓励调整叶形状的新基因的探索。在这研究,印射的基因分析和基因为从装饰用的梨树变化 Zhonghua 11 识别的新奇滚动叶异种被执行。SSRmarker 分析证明异种被位于染色体 9 的单个后退的基因(rl_(9 (t))) 控制。Rl_(9 (t)) 印射地点的罚款与标记在 Rl_(9 (t)) 附近开发了的 30 新圣被进行抛锚的区域基于在 Nipponbare and93-11 之间的顺序差异。印射的罚款要求了包含 Rl_(9 (t)) 的 PAC contig 的 contruction 地点,它被限定到 42 kb 区域。这能因此提高在进一步的研究克隆目标基因。
Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf shape. In this study, genetic analysis and gene mapping were carried out for a novel rolling leaf mutant identified from japonica variety Zhonghua 11. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (rl9(t) located on chromosome 9. Fine mapping of the Rl9(t) locus was conducted with 30 new STS markers developed around Rl9(t) anchored region based on the sequence diversity between Nipponbare and 93-11. The fine mapping necessitated the contruction of a PAC contig encompassing the Rl9(t) locus, which was delimited to a 42 kb region. This could therefore enhance the cloning of the target gene in further studies.