中文摘要：

利用植物的诱导抗性防治病虫害越来越受重视,为寻求枸杞害虫防治的新方法,探索外源茉莉酸对枸杞的诱导反应及机制,本研究测定了外源茉莉酸（jasmonic acid,JA）及枸杞瘿螨Aceria pallida Keifer危害对枸杞叶蛋白酶抑制剂（protease inhibitors,PIs）、超氧化物歧化酶（superoxide dismutase,SOD）、苯丙氨酸解氨酶（phenylalanine ammonia-lyase,PAL）活性及多糖（polysaccharide）、木质素（xylogen）含量的变化。结果表明：外源JA处理或枸杞瘿螨危害均使枸杞叶PIs,SOD和PAL活性及木质素含量显著提高,却使多糖含量显著降低。胰凝乳蛋白酶抑制剂（chymotrypsin inhibitors,CI）和胰蛋白酶抑制剂（trypsin inhibitor,TI）在喷洒JA或枸杞瘿螨危害的第5天增幅最大,且在JA浓度、瘿螨危害间差异显著（P〈0.05）,其中,0.1mmol/L JA处理使CI增加了96%,TI增加了128%,枸杞瘿螨危害使CI增加了94%,TI增加了122%。枸杞叶多糖含量在JA处理或瘿螨危害后显著降低（P〈0.05）,且与JA浓度有关,浓度越高,降低越明显。JA或瘿螨危害使枸杞叶木质素增加的最大值出现在处理后的第5天,高浓度JA、瘿螨危害及低浓度JA分别使其增加了70%,41%和36%,三者之间差异显著（P〈0.05）,第10天增加幅度减低。3种处理同样使SOD和PAL活性显著增加（P〈0.05）,第5天时增幅最大,高浓度JA处理使枸杞叶SOD活性由对照的70.77U增加到128.98U,PAL活性由10.91U增加到20.59U,第10天时呈现下降趋势。这些与抗性有关物质的变化及其变化趋势,说明外源茉莉酸及枸杞瘿螨均可诱导枸杞产生防御反应,而且反应的程度与茉莉酸浓度及处理后的时间有关。

英文摘要：

More and more attention was paid to the induced resistance in plant pest control. In order to explore a new control method for wolfberry pests, the induced defense response in wolfberry and the defense mechanism were studied. The changes of activities of protease inhibitors （ PIs ）, superoxide dismutase （SOD） and phenylalanine ammonia-lyase （PAL）, and the contents of polysaccharide and xylogen in wolfberry （Lycium barbarum） leaves subjected to exogenous JA treatment and damage by gall mite Aceria pallida were tested. The results showed that the activities of PIs, SOD and PAL and the xylogen content increased significantly in wolfberry leaves subjected to treatments with JA at the concentrations of 0. 001 and 0.1 mmol/L and the damage by A. pallida compared with the control not subjected to JA treatment and the damage by A. pallida, but the polysaccharide content was dramatically reduced. The JA treatment or the damage by A. pallida caused significant increase in activities of chymotrypsin inhibitors （CI） and trypsin inhibitors （TI） and the change extent differed among the three treatments （two JA treatments and one damage treatment by A. pallida） （ P 〈 0. 05 ）. Activities of CI and TI increased 96% and 128%, respectively, in wolfberry leaves treated with 0.1 mmol/L JA, and 94% and 122%, respectively, in wolPnerry leaves damaged by A. paUida. The polysaccharide content in wolfberry leaves treated with JA or gall mite reduced significantly （ P 〈 0.05 ）, and the reduction was related with the JA concentrations, the higher the JA concentration, the less polysaccharide content. The xylogen content increased significantly in wolfberry leaves treated by 0. 1 mmol/L JA, gall mite and0. 001 mmol/L JA （P 〈0.05） , and reached the peak at 5 d after treatment, which increased by 70% , 41% and 36%, respectively. The activities of SOD and PAL increased significantly in wolfberry leaves treated by JA or gall mite （ P 〈 0.05 ）, and also reached the peak at 5 d after treatment. T