目的:研究狗脊中鞣质类成分的纯化工艺。方法:以大孔吸附树脂纯化狗脊中鞣质类成分。以静态吸附量、静态吸附率和静态解吸量、静态解吸率为指标,筛选大孔吸附树脂型号;以洗脱率为指标,筛选纯化工艺。结果:最佳纯化工艺为选用D101型大孔吸附树脂,上样量为6个柱体积(BV),洗脱流速为1BV/h,3BV水除杂,各收集4BV的10%和20%乙醇洗脱液。该工艺下,洗脱率为92.64%,总鞣质质量分数为69.5%。结论:所选工艺合理、可行,可作为狗脊中鞣质类成分的纯化工艺。
OBJECTIVE:To study the purification technology of total tannins from Cibotium baronetz.METHODS:Tannins from C.baronetz was optimized by macroporous adsorptive resins.The types of macroporous adsorptive resins were screened with static adsorption capacity,static adsorption rate,static desorption quantity and static desorption rate as index.The purification technology was optimized with elution rate as index.RESULTS:The optimal purification technology was as follows:D101 macroporous adsorption resin,sample of 6 BV,flow rate of 1 BV/L,eluted by 3 BV water,collecting 10% and 20% ethanol eluent of 4 BV.The elution rate was 92.64% and the content of total tannins was 69.5%.CONCLUSION:The technology is reasonable and feasible,and it can be used for the purification technology of tannins from C.baronetz.