中文摘要：

调查在氮的氧化物 synthases (eNOS ) G894T 和 27 bp 变量数字双人脚踏车重复的 endothelial (VNTR ) 之间的关系的目的在中国汉国籍的绝经后的女人的基因多型性和骨质疏松症。在礼品的方法学习，来自在韦斯特中国的西安城市的区域的 281 个绝经后的女人被招募，并且根据世界健康组织建议的骨质疏松症的诊断标准划分了成骨质疏松症， osteopenia，和正常的组() 。腰部的 vertebrae 和左臀部的骨头矿物质密度(BMD ) 价值被 QDR-2000 双精力 X 光检查 absorptiometry 决定。血样品为包括睾丸激素， estradiol， calcitonin， osteocalcin，和职业人员骨胶原的生物化学的指示物打的血浆被测试我由连接酶的免疫吸着剂试金(ELISA ) 的氨基终端的职业人员肽，由 spectrophotometric 方法的酒石酸盐抵抗的酸磷酸酶，和由 Griess 的氮的氧化物的内容方法。染色体 DNA 从整个血被提取，并且 eNOS 基因的 G894T 多型性被使用碎片长度多型性(PCR-RFLP ) 方法和 eNOS 基因的 27 bp-VNTR 多型性是的聚合酶链反应限制分析由 PCR 的 genotyped 方法。然后，在遗传型和生物化学的指示物，遗传型和骨质疏松症之间的关系，和 haplotypes 和骨质疏松症被分析。平均 BMD 大腿骨的颈，病房的三角和腰部的 vertebrae 珍视的结果在有在 eNOS G894T 地点的 T/T 遗传型的题目的 1 ∼4 (L1∼L4 ) 比在有 G/T 和 G/G 遗传型(P【0.05 ) 的题目的那些显著地高。在有 eNOS 的 a/a 遗传型的题目的大腿骨的颈的平均 BMD 27 bp-VNTR 地点在有 b/b 遗传型(P【0.05 ) 的题目比那显然高。在 eNOS G894T G/T 遗传型的题目的血浆睾丸激素和 osteocalcin 集中比在另外的遗传型(P【0.05 ) 的题目的那些显然高；在 eNOS 的题目的血浆 estradiol 集中 27 bp-VNTR a/a 遗传型在 b/b 遗传型(P【0.01 ) 的题目显然比那高。在骨质疏松症女人， osteopenia 女人，和正常女人的 eNOS

英文摘要：

Objective： To investigate the relationships between endothelial nitric oxide synthases （eNOS） G894T and 27 bpvariable number tandem repeat （VNTR） gene polymorphisms and osteoporosis in the postmenopausal women of Chinese Han nationality. Methods： In the present study, 281 postmenopausal women from Xi＇an urban area in West China were recruited, and divided into osteoporosis, osteopenia, and normal groups according to the diagnostic criteria of osteoporosis proposed by World Health Organization （WHO）. The bone mineral density （BMD） values of lumbar vertebrae and left hips were determined by QDR-2000 dual energy X-ray absorptiometry. Blood samples were tested for plasma biochemical indicators including testosterone, estradiol, calcitonin, osteocalcin, and procollagen type I amino-terminal propeptide by enzyme-linked immunosorbent assay （ELISA）, tartrate-resistant acid phosphatase by spectrophotometric method, and the content of nitric oxide by Griess method. Genome DNA was extracted from whole blood, and G894T polymorphism of eNOS gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method and 27 bp-VNTR polymorphism of eNOS gene was genotyped by PCR method. Then the relationships between genotypes and biochemical indicators, genotypes and osteoporosis, and haplotypes and osteoporosis were analyzed. Results： The average BMD values of the femoral neck, ward＇s triangle and lumbar vertebrae 1-4 （LI-L4） in the subjects with T/T genotype in eNOS G894T locus were significantly higher than those in the subjects with G/T and G/G genotypes （P〈0.05）. The average BMD of the femoral neck in the subjects with a/a genotype of eNOS 27 bp-VNTR locus was evidently higher than that in the subjects with b/b genotype （P〈0.05）. The plasma testosterone and osteocalcin concentrations in the subjects of eNOS G894T G/T genotype were evidently higher than those in the subjects of other genotypes （P〈0.05）; the plasma estradiol concentra