利用EMS(ethylmethane sulfonate)诱变粳稻品种日本晴,筛选到一个能稳定遗传的少蘖直立穗突变体ltep1(low tiller number and erect panicle 1)。该突变体成熟期穗部保持直立,且分蘖减少。ltep1突变体的穗轴壁和茎秆壁较野生型厚,壁厚与外径之比变大。遗传分析表明,该突变表型受隐性单基因控制。利用图位克隆法将ltep1基因定位于水稻第10染色体长臂SSR标记bep16和RM25866之间160kb的区域内,该区域内尚未发现与水稻穗和分蘖发育相关的基因。
A novel rice mutant named ltep1(low tiller number and erect panicle 1)was obtained from the wild type Nipponbare after EMS treatment.It was mainly characterized by erect panicles at the maturity stage with reduced tillers.The results showed that both rachis wall and culm wall in the ltep1 mutant were thicker than those in the wild-type plant,providing the mutant with stronger mechanical endurance.Genetic analysis indicated that the abnormal phenotype was controlled by a single recessive gene.By map-based cloning,ltep1 was mapped in a 160 kb region between two SSR markers,bep16 and RM25866,at the long arm of chromosome 10.Since no homologous gene had been found in this region,ltep1 might be a novel gene related to the development of rice panicle and tiller.