中文摘要：

目的 探讨超高分子量聚乙烯（UHMWPE）颗粒对巨噬细胞的影响及颗粒对转录因子活化T细胞核因子-2（NFAT2）表达的影响。方法2004年11月至2005年2月将UHMWPE颗粒与巨噬细胞共同培养，在不同时段内观察细胞吞噬UHMWPE颗粒的情况，检测细胞增殖活性的变化，分别采用免疫组化和RT-PCR法检测钙调磷酸酶（CaN）和NFAT2表达的变化。结果巨噬细胞早期即开始吞噬UHMWPE颗粒，CaN和NFAT2表达增强，但细胞增殖活性不增加。结论UHMWPE颗粒能够显著引起巨噬细胞的吞噬，同时使细胞内转录因子NFAT2表达增强。

英文摘要：

Objective To study the effect of ultra high molecular weight polyethylene （UHMWPE） particles on macrophages and evaluate the expression of NFAT2, a key transcriptional factor for osteoclast differentiation. Methods From November 2004 to February 2005, macrophages were co-cultured with UHMWPE particles. When observed at different times, the proliferation activity of macrophages was analyzed by MTT and the expression of calcineurin （CaN） and NFAT2 by immunohistochemical and RT-PCR method respectively. Results The macrophages phagocytosed UHMWPE particles in an early time, the expression of CaN and NFAT2 was increased, while the proliferation activity was not enhanced. Conclusions UHMWPE particles can stimulate macrophages to phagocytose significantly, and enhance the expression of the transcriptional factor NFAT2.