中文摘要：

本试验旨在考察饲粮添加精氨酸（arginine,Arg）对免疫应激仔猪生长性能和肠道组织细胞膜外Toll样受体（TLR2、TLR4、TLR5、TLR6）基因表达的影响。选用（24±1）日龄的断奶、平均体重（7.19±0.63）kg的杜洛克×长白×大白公猪20头,随机分为4个处理,分别为饲喂基础饲粮＋注射生理盐水（对照组）、饲喂基础饲粮＋注射沙门氏杆菌活疫苗（S.C500）（S.C500组）、饲喂基础饲粮添加0.5%Arg＋注射S.C500（0.5%Arg组）和饲喂基础饲粮添加1.0%Arg＋注射S.C500（1.0%Arg组）,每个处理5个重复,每个重复1头仔猪。应激处理于第8天实施,肌肉注射S.C500 4 mL。试验期17 d。第8（免疫应激前）、9、11、18天采集血样测定血清沙门氏菌抗体水平和IL-6浓度,第18天仔猪屠宰采集空肠和回肠组织样品,测定Toll样受体mRNA表达量。结果表明：与对照组相比,仔猪肌肉注射S.C500不同程度地降低了平均日增重（ADG）（P〈0.10）和平均日采食量（ADFI）（P〈0.05）,显著增加了在第9、11和18天血清IL-6浓度（P〈0.05）,同时显著上调了空肠和回肠组织中TLR4和TLR5 mRNA表达量（P〈0.05）;添加Arg（0.5%和1.0%）能够缓减疫苗注射导致的以上抑制的效应,显著抑制了仔猪血清IL-6浓度、空肠和回肠TLR4和TLR5 mRNA表达量的上调（P〈0.05）。结果提示,饲粮添加Arg能显著减缓因S.C500注射引起的断奶仔猪肠道TLR4和TLR5基因的过度表达、血清IL-6浓度的升高,从而缓解免疫应激对仔猪的损伤。

英文摘要：

This trial was conducted to study the effect of arginine（Arg） supplementation on growth performance and genes expression of cell-surface toll-like receptors（TLR2,TLR4,TLR5 and TLR6） in intestinal tissues of weaned piglets under immune stress.Twenty male weaned piglets [（Duroc×Landrace×Yorkshire）,（24±1） days of age,average body weight of（7.19±0.63） kg] were randomly assigned to 4 treatments with 5 replicates in each treatment and 1 piglet per replicate.Piglets were fed basal diet＋injected sterile saline（control group）,fed basal diet＋injected live vaccine of Salmonella choleraesuis 500（S.C500）（S.C500 group）,fed basal diet supplemented with 0.5% Arg＋injected S.C500（0.5% Arg group）,and fed basal diet supplemented with 1.0% Arg＋injected S.C500（1.0% Arg group）,respectively.The injection of 4 mL S.C500 or sterile saline was carried out on day 8.The trial lasted for 17 d.Blood samples were collected to determine serum Salmonella antibody level and interleukin-6（IL-6） concentration on day 8（before injection）,day 9,day 11 and day 18.Piglets were slaughtered and jejunum and ileum tissue samples were collected for evaluation of mRNA expression levels of toll-like receptors on day 18.The results showed as follows： compared with the control group,injection of S.C500 decreased the ADG（P0.10） and ADFI（P0.05） of piglets to a different extent,significantly increased serum IL-6 concentration on day 9,day 11 and day 18（P0.05）,as well as mRNA expression levels of TLR4 and TLR5 in jejunum and ileum tissue（P0.05）;Arg supplementation（0.5% and 1.0%） alleviated inhibitive effects induced by S.C500 challenge,decreased serum IL-6 concentration,and inhibited mRNA expression levels of TLR4 and TLR5 in jejunum and ileum tissue（P0.05）.These results indicate that Arg supplementation can alleviate the over-expression of TLR4 and TLR5 mRNA in intestinal tissues and the excessive production of serum IL-6 induced by S.C500 challenge,which are beneficial to alleviat