中文摘要：

目的：建立一种新的植入式颅骨电极记录大鼠视觉诱发电位（visual evoked potential，VEP）的方法，同时应用于RCD大鼠及CSNB大鼠，看是否可行。方法：大鼠麻醉后，暴露颅骨表面，分别在前囟（bregma）前4mm和后8mm处钻孔，植入长2mm，直径1．2mm的不锈钢螺钉，分别作为参考和记录电极；接地电极接入尾部。记录暗适应和明适应下视觉诱发电位（刺激光强：0．011，0．035，0．11，0．35，1．1和3．5ed·s／m^2，叠加100次，不同光强间隔2～5min，刺激频率1Hz，时程300ms）。1wk后，以同样条件再次记录暗适应和明适应下视觉诱发电位。结果：植入电极后的大鼠存活时间较长，能达数月。记录时基本无干扰，波形清晰，稳定。植入法记录的波形与传统方法记录（记录电极捕于两耳连线中点，向前刺入1～1．5cm，保持电极与身体长轴平行；参考电极由不锈钢针制成，插入右侧颊部；接地电极也由不锈钢针制成，置于尾部，对侧眼遮盖。）的波形没有差异，但幅值明显增大。在视锥细胞缺失（RCD）及先天性静止性夜盲（CSNB）大鼠中得到了同样的结果。结论：由于电极可以长期留置于大鼠上，有利于观察其视觉诱发电位的动态改变，对长期病程变化的研究也非常有利，同时记录和参考电极的位置同定，两者之间的相对位置也无变化，可大大减少实验误差，提高结果的可重复性及精确性。

英文摘要：

AIM ： To establish a new method to record visual evoked potential of rats and this method was applied to retinal cone degeneration （RCD） and congenital stationary night blindness （CSNB） rats to see whether it is feasible. METHODS： After anesthesia, the rats＇ scalp surface was exposed and two stainless screws were implanted at 4mm in front of and 8mm behind Bregma. The front one was reference electrode and the latter one was recording electrode. The ground electrode was joined to the tail. And we recorded VEP of scotopic adaptation and photopic adaptation （The stimulus intensity of light ： 0.011, 0.035, 0.11,0.35,1.1 and 3.5cd·s/m^2. They were all overlapped for 100 times. There were 2-5 minutes intervals between different intensities. The frequency of stimulation was 1 Hz and time course was 300ms）. A week later, we recorded the VEP again in the same conditions. RESULTS： The rats who were implanted with electrodes could live longer. When they were recorded, there was no disturbance and the waveforms were very clear and steady. There was no difference between the waveforms recorded by conventional method and those by implanted electrodes while the amplitude of new method was larger than conventional. We could get the same conclusion in RCD and CSNB rats. CONCLUSION： Since the electrodes could be kept on the scalp for a longer time, we could not only observe dynamic changes of VEP but also observe the long-duration pathological changes in the rats. Because the positions of recording and reference electrodes were fixed, the system error could be reduced and the repeatability of experiments increased.