中文摘要：

建立了一种简单、可靠的空间温度梯度芯片毛细管电泳DNA突变分析系统,制作了热阻呈梯度均匀变化的硅橡胶（PDMS）基片,利用其热阻变化对热传导的影响,在基片表面形成稳定的空间温度梯度.通过改变PDMS基片的厚度差,可得到范围不同的温度梯度,且形成的温度梯度在6h内保持稳定.利用该温度梯度加热装置对玻璃微流控芯片进行加热,在10℃温度梯度范围内对209bp的DNA突变标准样品进行分离检测,单次样品分析时间为8.3min,并成功用于3例大肠癌患者石蜡组织切片中K-ras基因突变的检测.

英文摘要：

A simple and reliable on-chip spatial temperature gradient capillary electrophoresis was developed for DNA mutation detection using a thermal resistance gradient heating system.Polydimethylsiloxane（PDMS） slab with thermal resistance varying in gradient was made,and continuous temperature gradient was formed on slab surface using a single thermostated copper heater block owing to heat exchange influenced by varied thermal resistance.Different temperature gradients were produced by regulating the thickness of PDMS slab,and the temperature gradients were stable and uniform within 6 h.Utilizing the heating system,209 bp DNA mutation standard samples were separated within a wide temperature range of 10℃on a glass microchip with an assay time of 8.3 min.Effectiveness of the system was demonstrated by the successful detection of K-ras gene mutations in paraffin tissue sections from 3 colorectal cancer patients.