脂质体是类脂分子的自组装体,是一种由一个或多个脂双层中间包覆微水相的结构,是生物膜的良好模型.1995年,Zhang等首先将脂质体用于毛细管电泳(CE)并考察了不同药物与脂质体间的相互作用,创立了新的脂质体毛细管电泳(Liposome capillaryel ectrophoresis,LCE).目前,LCE主要用于化合物的分离分析,而用于测定化合物疏水参数的报道则不多见。
Liposome capillary electrophoresis(LCE) provides a novel and facile approach for determining hydrophobic parameters(lgP) of organic compounds. In order to avoid the error of the migration time of liposome phase(t1) on determination, a novel technique was developed in this paper, in which the t1 was obtained via non-linearity fitting with lgP values from literatures and migration time(tm) of a series of standard compounds. lgP values of the six benzene derivatives determined by this LCE method were compared with literature values and the ones obtained by directly determined t1 and MEEKC. The average error between lgP values determined by two LCE methods was 0. 07 logarithm units. The LCE method was simple and rapid, which can provide a new way to determine lgP of organic compounds.