中文摘要：

目的：探讨幽门螺杆菌（H P ）感染相关胃炎程度与胃黏膜细胞凋亡基因表达的相关性。方法将2013年11～12月大坪医院消化内镜中心45例慢性胃炎患者纳入研究，快速尿素酶实验和13 C-呼气实验检测H P感染情况，病理分析H P感染部位胃炎严重程度，实时定量PCR （qRT-PCR）法检测HP感染部位胃黏膜组织和无 HP感染的胃黏膜组织中凋亡相关基因Bax、Bak、Bcl-2的表达，Person相关分析 HP感染的慢性胃炎程度与凋亡基因表达的相关性。结果45例患者13 C-呼气实验和胃窦黏膜快速尿素酶实验均阳性，提示H P感染。病理结果显示45例（100％）患者均为慢性胃窦胃炎，其中28例（62．2％）为轻度，16例（35．6％）为中度，1例（2．0％）为重度；9例（20．0％）肠化生，5例（11．1％）低级别上皮内瘤变。45例患者胃体黏膜尿素酶实验阴性，提示无 HP感染，其中6例胃体组织显示轻度慢性炎症（13．3％），39例无炎症（86．7％）；无肠化生及上皮内瘤变。qRT-PCR结果显示HP感染胃黏膜中Bax mRNA的表达明显高于 HP未感染胃黏膜患者（P＜0．01）且与胃炎程度呈明显正相关（P＜0．01），而Bak和Bcl-2的表达在两组间差异无统计学意义（ P＞0．05）。结论 H P感染的慢性胃炎程度与胃黏膜细胞凋亡呈正相关，提示H P感染可能导致胃上皮细胞中Bax基因的表达从而促进细胞凋亡。

英文摘要：

Objective To investigate the correlation between Helicobacter pylori （HP） infection-associated gastritis and the ap-optotic genes in gastric mucosa .Methods Forty-five patients with chronic gastritis were registrated in our study from November 2013 to December 2014 .HP infection status in the patients was detected by using urease test and 13C-urea breath test .The degree of gastritis in the gastric mucosa with HP infection was confirmed via histopathology .qRT-PCR was used to measure the mRNA ex-pressions of Bax ,Bak and Bcl-2 in the gastric mucosa with HP infection and matched normal gastric mucosa .Person analysis was used to assess the correlation between the HP infection-associated gastritis and the mRNA expressions of Bax ,Bak and Bcl-2 in the gastric mucosa .Results Forty-five patients with HP infection in antrum and 45 patients （100% ） with chronic antrum gastritis were identified ,including 28 patients （62 .2% ） with light gastritis ,16 patients （35 .6% ） with moderate gastritis ,1 patient （2 .0% ） with severe gastritis .9 patients （20 .0% ）with metaplasia ,5 patients（11 .1% ） with low grade intraepithelial neoplasms .The urease tests were negative in the gastric body of 45 patients ,6 patients （13 .3% ）were mild chronic gastritis in the body ;Patient with meta-plasia and intrapithelial gastritis was not found .The Bax expression in the HP-infected gastric mucosa was markedly increased when compared with the normal gastric mucosa （P〈 0 .01） ,and positively correlated with the degree of gastritis （P〈 0 .01） , whereas the expressions of Bak and Bcl-2 have no significantly deferences bttween two groups（P〉0 .05） .Conclusion HP infec-tion-associated gastritis positively correlated with the expressions of apoptotic genes in gastric mucosa ,suggesting that HP infection might result in increasing the Bax expression and further enhancing the cell apoptosis .