中文摘要：

目的研究中国南海蜂海绵属Haliclona cymaeformis的化学成分。方法运用正相硅胶、反相硅胶（ODS）和凝胶（Sephadex LH-20）等多种柱色谱手段对化合物进行分离纯化;通过理化性质和波谱数据,结合文献对照,鉴定化合物的结构。结果从海绵H.cymaeformis的乙醇提取物中共分离鉴定了11个单体化合物：24-亚甲基胆甾醇（1）,5α,8β-epidioxy-cholesta-6-en-3-ol（2）,cholesta-7-en-3β,5α,6β,9α-tetraol（3）,胆甾醇（4）,3-吲哚甲酸（5）,尿嘧啶（6）,胸腺嘧啶（7）,尿嘧啶核苷（8）,胸腺嘧啶核苷（9）,尿嘧啶脱氧核苷（10）和胸腺嘧啶脱氧核苷（11）。结论化合物1~11均为首次从该海绵中分离得到。细胞毒活性测试结果表明,化合物3对慢性髓原白血病细胞（K562）和人肝癌细胞（SMMC-7721）的增殖显示了较强的生长抑制活性。

英文摘要：

Objective To study the chemical constituents of the sponge Haliclona cymaeformis collected from the South China Sea.Methods The compounds were isolated and purified by column chromatography on silica gel,reversed-phase silica gel（ODS） and Sephadex LH-20,and their structures were elucidated on the basis of physicochemical and spectral analysis.Results Eleven compounds were isolated from the ethanol extract of H.cymaeformis,and their structures were identified as 24-methylene-cholesta-3β-ol（1）,5α,8β-epidioxy-cholesta-6-en-3-ol（2）,cholesta-7-en-3β,5α,6β,9α-tetraol（3）,cholesterol（4）,1H-indole-3-carboxylic acid（5）,uracil（6）,thymine（7）,uridine（8）,thymidine（9）,2＇-deoxyuridine（10）,and 2＇-deoxythymidine（11）,respectively.Conclusion All the isolated compounds were obtained from the sponge H.cymaeformis for the first time.Compounds 3 showed significant inhibitory activities towards chronic myelogenous leukemia（K562） and human hepatoma（SMMC-7721） cell lines.