中文摘要：

目的比较烟曲霉生物被膜（BF）在体外流动与静止模型中的成膜能力,获取较佳的体外研究模型,为烟曲霉今后的体外研究提供技术方法支持。方法分别建立烟曲霉生物被膜体外流动模型和静止模型,在生物被膜形成的不同阶段行FITC-Canavalia ensiformis（FITC-ConA）胞外基质（ECM）染色,激光扫描共聚焦显微镜（CLSM）观察烟曲霉生物被膜三维（3D）形态,COMSTAT软件定量分析3D图像堆,比较两种培养状态下生物被膜的生物量、平均厚度等生长指标。结果在静止模型和流动模型中,BF的生物量、平均厚度、基质覆盖率、平均扩散距离均随着培养时间的延长而升高,粗糙系数、表面积与生物量比随着培养时间的延长而降低;相同时间点下,静止模型组形成的BF的生物量、平均厚度、基质覆盖率、平均扩散距离较流动模型组的高,差异有统计学意义（P〈0.05）;静止模型组粗糙系数和表面积与生物量比较流动模型组的低,前者差异有统计学意义（P〈0.05）,后者差异无统计学意义。结论烟曲霉在流动模型中亦可形成稳定的生物被膜,但静止模型可分泌更多的ECM,形成更厚的生物被膜,更利于研究者对体外烟曲霉BF进行研究。

英文摘要：

OBJECTIVE To compare the biofilm（BF）formation ability of Aspergillus fumigatus between flow chamber system and static system in vive,to find out the best external study model,so as to provide technical support to external study.METHODS There were two in vitro biofilm models of A.fumigatus established：flow chamber system model and static model,which were stained the extracellular matrix（ECM）with FITC-Canavalia ensiformis（FITC-ConA）at different time points,and their three-dimensional（3D）biofilm morphology with laser scanning confocal microscope（CLSM）were observed.The 3Dimage stacks were under quantitatively analysis with COMSTAT software to compare the growth index（e.g.biomass and average thickness）between two groups.RESULTS Within each model,biomass,average thickness,substrate coverage,and the average diffusion distance increased according to time period,while roughness coefficient,surface area to biomass ratio decreases decreased with time;between groups,biomass,average thickness,substratum coverage and average diffusion of BF formed in static model were significantly higher than that of continuous flow system under the same time point（P〈0.05）,while roughness coefficient,surface to biovolume ratio were significantly lower（P〈0.05）,but the following one had not.CONCLUSION A.fumigatus can form biofilm stably in flow system,but the biofilm under static system can secrete more ECM and form a thicker biofilm,which is more appropriate for A.fumigatus biofilm studies in vitro.