中文摘要：

比较了人工汰选的对Cry1Ab杀虫蛋白产生抗性的亚洲玉米螟（Ostrinia furnacalis（Guenées））种群和敏感种群幼虫解毒酶和中肠蛋白酶的变化。结果表明：抗、感种群的乙酰胆碱酯酶、中肠总蛋白酶、弱碱性类胰蛋白酶和类胰凝乳蛋白酶的活力差异不显著，而抗性种群的α-乙酰萘酯酶和类胰蛋白酶显著高于敏感种群。对类胰蛋白酶、类胰凝乳蛋白酶和类弹性蛋白酶的活力杂交分析结果表明，抗性种群的类胰蛋白酶酶谱强于敏感种群，但抗、感种群中肠液在体外对Cry1Ab原毒素的酶解过程并不存在差异。推断强碱性类胰蛋白酶可能与亚洲玉米螟对Bt抗性产生机理有关。

英文摘要：

Both detoxification enzymes and midgut proteinase activities of Bacillus thuringiensis resistant and susceptible Ostrinia furnaclis were compared. The results showed that the differences of the activity of the acetylcholinesterase, the total protease, the weak alkaline trypsin-like proteinase, and the chymotrypsin-like proteinase between the larvae of susceptible and resistant strains were no significant However, the activities of the α-naphthylacetate esterase and the active alkaline trypsin-like proteinase from larvae of Bt resistant strain were higher than those in the susceptible strain. Activity blotting analysis of trypsin-, chymotrypsin-, and elastase-like proteinase was conducted. The band＇s intensities of the trypsin-like proteinase in the resistant strain were stronger than that in susceptible swain. No difference was observed in the Cry1Ab protoxin hydrolysis processing by proteinase extracts from the susceptible and resistant strains. Active alkaline trypsin-like enzyme may be associated with the development of resistance to Bt toxin of the Asian corn borer.